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Tytuł:
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Research Note: All-trans retinoic acids induce adipogenic differentiation of chicken embryonic fibroblasts and preadipocytes.
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Autorzy:
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Kim DH; Department of Animal Sciences, The Ohio State University, Columbus, OH, USA.
Lee J; Department of Animal Sciences, The Ohio State University, Columbus, OH, USA; The Ohio State University Interdisciplinary Human Nutrition Program, The Ohio State University, Columbus 43210, USA.
Suh Y; Department of Animal Sciences, The Ohio State University, Columbus, OH, USA.
Cressman M; Department of Animal Sciences, The Ohio State University, Columbus, OH, USA.
Lee K; Department of Animal Sciences, The Ohio State University, Columbus, OH, USA; The Ohio State University Interdisciplinary Human Nutrition Program, The Ohio State University, Columbus 43210, USA. Electronic address: .
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Źródło:
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Poultry science [Poult Sci] 2020 Dec; Vol. 99 (12), pp. 7142-7146. Date of Electronic Publication: 2020 Sep 12.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
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MeSH Terms:
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Adipogenesis*/drug effects
Chickens*
Tretinoin*/pharmacology
Animals ; Cell Differentiation/drug effects ; Cells, Cultured ; Chick Embryo ; Female ; Fibroblasts/drug effects
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References:
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Contributed Indexing:
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Keywords: adipogenesis; all-trans retinoic acid; chicken; chicken embryonic fibroblasts; preadipocytes
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Substance Nomenclature:
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5688UTC01R (Tretinoin)
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Entry Date(s):
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Date Created: 20201129 Date Completed: 20210412 Latest Revision: 20210412
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Update Code:
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20240105
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PubMed Central ID:
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PMC7704976
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DOI:
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10.1016/j.psj.2020.09.006
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PMID:
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33248631
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Adipocytes store excess energy in the form of lipids, whereas fat accretion contributes to feed efficiency, meat quality, and female reproduction in poultry. As a metabolite of vitamin A, all-trans retinoic acid (atRA) has been shown to have influence over metabolic functions such as lipid and energy homeostasis, as well as adipogenesis. Although atRA has been known to function as a regulating factor in mammalian adipogenesis, the effects of atRA on adipogenesis has not been studied in chickens. In this study, chicken preadipocytes isolated from leg fat tissues at embryonic day (E) 14 and chicken embryonic fibroblasts (CEF) harvested at E5 were cultured. The preadipocytes and CEF in culture with 10% chicken serum were treated with various concentrations (0 μmol, 100 μmol, or 150 μmol) of supplemented atRA for 48 h. In these cells, cytoplasmic lipid droplet accumulation and mRNA expression for adipogenic genes were analyzed by Oil-Red-O staining and quantitative real-time PCR, respectively. Analysis of the relative amount of Oil-Red-O staining (lipid accumulation) revealed that all 3 variables increased in a dose-dependent manner, in response to increasing atRA supplementation. Genes involved in adipocyte differentiation, fatty acid transport, and triacylglycerol synthesis in both E14 preadipocytes and E5 CEF were upregulated by supplementation of atRA. These data demonstrated that atRA alone promoted adipogenesis of embryonic preadipocytes and fibroblasts in vitro, suggesting that atRA has an influential role in multiple stages of adipogenesis in chicken embryos.
(Copyright © 2020. Published by Elsevier Inc.)
