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Tytuł:
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Primary ciliary dyskinesia relative protein ZMYND10 is involved in regulating ciliary function and intraflagellar transport in Paramecium tetraurelia.
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Autorzy:
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Shi L; School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, 453003, PR China. Electronic address: .
Shen X; School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, PR China.
Chi Y; School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, PR China.
Shen Y; School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, PR China. Electronic address: .
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Źródło:
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European journal of protistology [Eur J Protistol] 2021 Feb; Vol. 77, pp. 125756. Date of Electronic Publication: 2020 Nov 22.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Stuttgart : Gustav Fischer Verlag
Original Publication: Stuttgart ; New York : G. Fischer, c1987-
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MeSH Terms:
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Cilia/*metabolism
Cytoskeletal Proteins/*genetics
Cytoskeletal Proteins/*metabolism
Paramecium tetraurelia/*genetics
Paramecium tetraurelia/*metabolism
Carrier Proteins/metabolism ; Cilia/genetics ; Cilia/pathology ; Mutation ; Protein Transport/genetics
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Contributed Indexing:
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Keywords: Cilia; Ciliogenesis; IFT; Paramecium; ZMYND10
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Substance Nomenclature:
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0 (Carrier Proteins)
0 (Cytoskeletal Proteins)
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Entry Date(s):
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Date Created: 20201206 Date Completed: 20210205 Latest Revision: 20210205
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Update Code:
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20240104
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DOI:
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10.1016/j.ejop.2020.125756
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PMID:
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33279757
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Cilia are highly conserved in most eukaryotes and are regarded as an important organelle for motility and sensation in various species. Cilia are microscopic, hair-like cytoskeletal structures that protrude from the cell surface. The major focus in studies of cilia has been concentrated on the ciliary dysfunction in vertebrates that causes multisymptomatic diseases, which together are referred to as ciliopathies. To date, the understanding of ciliopathies has largely depended on the study of ciliary structure and function in different animal models. Zinc finger MYND-type containing 10 (ZMYND10) is a ciliary protein that was recently found to be mutated in patients with primary ciliary dyskinesia (PCD). In Paramecium tetraurelia, we identified two ZMYND10 genes, arising from a whole-genome duplication. Using RNAi, we found that the depletion of ZMYND10 in P. tetraurelia causes severe ciliary defects, thus provoking swimming dysfunction and lethality. Moreover, we found that the absence of ZMYND10 caused the abnormal localization of the intraflagellar transport (IFT) protein IFT43 along cilia. These results suggest that ZMYND10 is involved in the regulation of ciliary function and IFT, which may contribute to the study of PCD pathogenesis.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)