Gui-A-Gra Attenuates Testicular Dysfunction in Varicocele-Induced Rats via Oxidative Stress, ER Stress and Mitochondrial Apoptosis Pathway.

Tytuł:: Gui-A-Gra Attenuates Testicular Dysfunction in Varicocele-Induced Rats via Oxidative Stress, ER Stress and Mitochondrial Apoptosis Pathway.
Autorzy:: Karna KK; Department of Urology, Institute for Medical Sciences, Jeonbuk National University Medical School, Jeonju 54907, Korea.; Biomedical Research Institute and Clinical Trial Center for Medical Device, Jeonbuk National University Hospital, Jeonju 54907, Korea.
Choi NY; Department of Urology, Institute for Medical Sciences, Jeonbuk National University Medical School, Jeonju 54907, Korea.; Biomedical Research Institute and Clinical Trial Center for Medical Device, Jeonbuk National University Hospital, Jeonju 54907, Korea.
Kim CY; College of Pharmacy, Hanyang University, Ansan 426791, Korea.
Kim HK; College of Pharmacy, Kyungsung University, Busan 48434, Korea.
Shin YS; Department of Urology, Institute for Medical Sciences, Jeonbuk National University Medical School, Jeonju 54907, Korea.; Biomedical Research Institute and Clinical Trial Center for Medical Device, Jeonbuk National University Hospital, Jeonju 54907, Korea.
Park JK; Department of Urology, Institute for Medical Sciences, Jeonbuk National University Medical School, Jeonju 54907, Korea.; Biomedical Research Institute and Clinical Trial Center for Medical Device, Jeonbuk National University Hospital, Jeonju 54907, Korea.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2020 Dec 03; Vol. 21 (23). Date of Electronic Publication: 2020 Dec 03.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Edible Insects*
Endoplasmic Reticulum Stress*/drug effects
Mitophagy*/drug effects
Oxidative Stress*/drug effects
Testis/*metabolism
Varicocele/*metabolism
Animals ; Apoptosis/drug effects ; Biomarkers ; Immunohistochemistry ; Inflammation Mediators/metabolism ; Lipid Peroxidation ; Male ; Mitochondria/metabolism ; Oxidoreductases/metabolism ; Rats ; Reactive Oxygen Species/metabolism ; Sperm Motility/drug effects ; Spermatozoa/metabolism ; Testis/drug effects ; Testis/pathology ; Varicocele/etiology ; Varicocele/pathology
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Grant Information:: 2392020G-N1 239bio Inc. Iksan, Chonbuk, South Korea
Contributed Indexing:: Keywords: Gui-A-Gra; apoptosis; endoplasmic reticulum (ER) stress; inflammation; mitochondria; oxidative stress; steroidogenic acute regulatory (StAR) protein; testis; varicocele
Substance Nomenclature:: 0 (Biomarkers)
0 (Inflammation Mediators)
0 (Reactive Oxygen Species)
EC 1.- (Oxidoreductases)
Entry Date(s):: Date Created: 20201208 Date Completed: 20210308 Latest Revision: 20210308
Update Code:: 20240104
PubMed Central ID:: PMC7730328
DOI:: 10.3390/ijms21239231
PMID:: 33287403
: Czasopismo naukowe

Pełny tekst

Gui-A-Gra, a commercial insect powder from Gryllus bimaculatus , is registered as an edible insect by the Korean food and drug administration. The aim of this study was to investigate the effect of Gui-A-Gra on testicular damage induced by experimental left varicocele in male Sprague Dawley rats. A total of 72 rats were randomly divided into the following six groups (12 rats in each group): a normal control group (CTR), a group administrated with Gui-A-Gra 1.63 gm/kg (G1.63), a group administrated with Gui-A-Gra 6.5 gm/kg (G6.5), a varicocele (VC)-induced control group (VC), a VC-induced group administrated with Gui-A-Gra 1.63 gm/kg (VC + G1.63), and a VC-induced group administrated with Gui-A-Gra 6.5 gm/kg (VC + G6.5). Rats were administrated 1.63 or 6.5 gm/kg Gui-A-Gra once daily for 42 days. Indicators of sperm parameters, histopathology, reproductive hormones, oxidative stress, endoplasmic reticulum (ER) stress, inflammation, and mitochondrial apoptosis were analyzed to evaluate effects of Gui-A-Gra on VC-induced testicular dysfunction. Gui-A-Gra administration to VC-induced rats significantly ( p < 0.05) increased sperm count and sperm motility, Johnsen score, spermatogenic cell density, serum testosterone, testicular superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, GPx4, and steroidogenic acute regulatory protein (StAR) level. Moreover, pretreatment with Gui-A-Gra significantly ( p < 0.05) decreased terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) positive cells/tubules, serum luteinizing hormone (LH), serum follicle-stimulating hormone (FSH), testicular tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), reactive oxygen species (ROS)/reactive nitrogen species (RNS) level, glucose-regulated protein-78 (Grp-78), phosphorylated c-Jun-N-terminal kinase (p-JNK), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1α (p-IRE1α), cleaved caspase-3, and BCL2 associated X protein: B-cell lymphoma 2 (Bax: Bcl2) ratio in VC rats. These results suggest that protective effects of Gui-A-Gra on VC-induced testicular injury might be due to its antioxidant, anti-inflammatory, and androgenic activities that might be mediated via crosstalk of oxidative stress, ER stress, and mitochondrial apoptosis pathway.

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