Responsive polymer-assisted 3D cryogel supports Huh7.5 as in vitro hepatitis C virus model and ectopic human hepatic tissue in athymic mice.

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Tytuł:: Responsive polymer-assisted 3D cryogel supports Huh7.5 as in vitro hepatitis C virus model and ectopic human hepatic tissue in athymic mice.
Autorzy:: Jayal P; Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India.
Behera P; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India.
Mullick R; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India.
Ramachandra SG; Centre for Animal Facility, Indian Institute of Science, Bangalore, Karnataka, India.
Das S; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India.
Kumar A; Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh, India.
Karande A; Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India.
Źródło:: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Mar; Vol. 118 (3), pp. 1286-1304. Date of Electronic Publication: 2020 Dec 25.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: <2005->: Hoboken, NJ : Wiley
Original Publication: New York, Wiley.
MeSH Terms:: Liver*/metabolism
Liver*/virology
Liver Transplantation*
Cryogels/*pharmacology
Hepacivirus/*metabolism
Hepatitis C/*metabolism
Alginates/chemistry ; Alginates/pharmacology ; Animals ; Disease Models, Animal ; Gelatin/chemistry ; Gelatin/pharmacology ; Heterografts ; Humans ; Mice ; Mice, Nude ; Polyethylene Glycols/chemistry ; Polyethylene Glycols/pharmacology
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Contributed Indexing:: Keywords: ectopic human liver; hepatitis C virus; hepatospheres; pH/temperature responsive polymers; polymeric cryogel
Substance Nomenclature:: 0 (Alginates)
0 (Cryogels)
3WJQ0SDW1A (Polyethylene Glycols)
9000-70-8 (Gelatin)
Entry Date(s):: Date Created: 20201209 Date Completed: 20220117 Latest Revision: 20220117
Update Code:: 20240104
DOI:: 10.1002/bit.27651
PMID:: 33295646
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The three-dimensional (3D) cell culture models serve as the interface between conventional two-dimensional (2D) monolayer culture and animal models. 3D culture offers the best possible model system to understand the pathophysiology of human pathogens such as hepatitis C virus (HCV), which lacks a small animal model, due to narrow host tropism and non-permissiveness of murine hepatocytes. In this study, functionally robust spheroids of HCV permissive Huh7.5 cells were generated, assisted by the temperature or pH-responsive polymers PNIPAAm and Eudragit respectively, followed by the long-term growth of the multilayered 3D aggregates in poly(ethylene glycol) (PEG)-alginate-gelatin (PAG) cryogel. The human serum albumin (HSA), marker of hepatic viability was detected up to 600 ng/ml on 24th day of culture. The 3D spheroid culture exhibited a distinct morphology and transcript levels with the upregulation of hepato-specific transcripts, nuclear factor 4α (HNF4α), transthyretin (TTr), albumin (Alb), phase I and phase II drug-metabolizing genes. The two most important phase I enzymes CYP3A4 and CYP2D6, together responsible for 90% metabolism of drugs exhibited up to 9- and 12-fold increment, respectively in transcripts. The 3D culture was highly permissive to HCV infection and supported higher multiplicity of infection compared to monolayer Huh7.5 culture. Quantitation of high levels of HSA (500-200 ng/ml) in circulation in mice for 32 days asserted integration with host vasculature and in vivo establishment of 3D culture implants as an ectopic human hepatic tissue in mice. The study demonstrates the 3D spheroid Huh7.5 culture as a model for HCV studies and screening potential for anti-HCV drug candidates.
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