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Tytuł pozycji:

Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular Biology.

Tytuł :
Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular Biology.
Autorzy :
Tang AAS; Astbury Centre for Structural and Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
Tiede C; Astbury Centre for Structural and Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
McPherson MJ; Astbury Centre for Structural and Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
Tomlinson DC; Astbury Centre for Structural and Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2247, pp. 105-121.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms :
Cell Biology*
Indicators and Reagents*
Molecular Biology*/methods
Carrier Proteins/*isolation & purification
Antibodies/metabolism ; Carrier Proteins/chemistry ; Cell Surface Display Techniques ; Cytological Techniques ; Enzyme-Linked Immunosorbent Assay ; Humans ; Peptide Library ; Protein Binding ; Structure-Activity Relationship
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Contributed Indexing :
Keywords: Affimer*; Artificial binding protein*; Inhibitor*; Panning*; Phage ELISA*; Phage display*; Protein–protein interaction*; Research reagents*
Substance Nomenclature :
0 (Antibodies)
0 (Carrier Proteins)
0 (Indicators and Reagents)
0 (Peptide Library)
Entry Date(s) :
Date Created: 20201210 Date Completed: 20210331 Latest Revision: 20210331
Update Code :
20210401
DOI :
10.1007/978-1-0716-1126-5_6
PMID :
33301114
Czasopismo naukowe
Artificial binding proteins have been validated as alternatives to antibodies in their use as research reagents in molecular and cellular biology. For example, they have been used as inhibitors of protein-protein interactions to modulate activity, to facilitate crystallization, and as probes for cellular imaging.Phage display is a widely used approach for isolating target-specific binding reagents, and it has even been used to isolate isoform-specific binding proteins and binders that can distinguish between highly homologous protein domains.Here, we describe methods that have been employed in isolating highly specific artificial binding proteins against a wide range of target proteins.

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