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Tytuł pozycji:

C8orf48 inhibits the tumorigenesis of colorectal cancer by regulating the MAPK signaling pathway.

Tytuł:
C8orf48 inhibits the tumorigenesis of colorectal cancer by regulating the MAPK signaling pathway.
Autorzy:
Lei L; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China. Electronic address: .
An G; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Zhu Z; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Liu S; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Fu Y; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Zeng X; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Cao Q; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Yan B; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
Źródło:
Life sciences [Life Sci] 2021 Feb 01; Vol. 266, pp. 118872. Date of Electronic Publication: 2020 Dec 09.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
MeSH Terms:
Gene Expression Regulation, Neoplastic*
MAP Kinase Signaling System*
Biomarkers, Tumor/*metabolism
Carcinogenesis/*pathology
Colorectal Neoplasms/*pathology
MicroRNAs/*genetics
Neoplasm Proteins/*metabolism
Apoptosis ; Biomarkers, Tumor/genetics ; Carcinogenesis/genetics ; Carcinogenesis/metabolism ; Cell Movement ; Cell Proliferation ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/metabolism ; Humans ; Neoplasm Invasiveness ; Neoplasm Proteins/genetics ; Prognosis ; Tumor Cells, Cultured
Contributed Indexing:
Keywords: C8orf48; Colorectal cancer; MAPK pathway; Metastasis; Proliferation
Substance Nomenclature:
0 (Biomarkers, Tumor)
0 (MIRN556 microRNA, human)
0 (MicroRNAs)
0 (Neoplasm Proteins)
Entry Date(s):
Date Created: 20201214 Date Completed: 20210201 Latest Revision: 20210201
Update Code:
20240104
DOI:
10.1016/j.lfs.2020.118872
PMID:
33309715
Czasopismo naukowe
Aims: Colorectal cancer (CRC) is a leading cause of cancer-related death globally. Thus, in this study, we aimed to investigate chromosome 8 open reading frame 48 (C8orf48) as a biomarker for early detection of CRC.
Main Methods: RNA expression and methylation profiles were downloaded from The Cancer Genome Atlas (TCGA) database. Cell proliferation, migration and invasion assays were performed to confirm the function of C8orf48 in CRC cells. Dual-luciferase reporter assay was used to identify that C8orf48 was the direct target of miR-556. Genomics of Drug Sensitivity in Cancer (GDSC) database, gene set enrichment analysis (GSEA) and western blot analysis were performed to explore the mechanism of C8orf48.
Key Findings: we found that C8orf48 is down-regulated in clinical samples of CRC tissues. Enrichment analysis showed that C8orf48 is associated with methylation biomarkers in CRC, and TCGA database confirmed that the methylation of C8orf48 is up-regulated in the early stage of CRC. We further revealed that the overexpression of C8orf48 decreased CRC cell proliferation, migration and invasion. Luciferase reporter indicated that C8orf48 was the direct target of the oncogene miR-556. Additionally, we used GDSC database, GSEA database and western blot analysis to demonstrate that C8orf48 plays a suppressor role in CRC by inhibiting MAPK signaling pathway.
Significance: C8orf48 was identified as a biomarker for early detection of CRC for the first time, and might provide novel information for CRC prediction and therapy.
(Copyright © 2020 Elsevier Inc. All rights reserved.)

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