Prevalence and virulence gene profiles of Escherichia coli O157 from cattle slaughtered in Buea, Cameroon.

Tytuł:: Prevalence and virulence gene profiles of Escherichia coli O157 from cattle slaughtered in Buea, Cameroon.
Autorzy:: Akomoneh EA; Department of Biological Science, Faculty of Science, University of Bamenda, Bamenda, Cameroon.
Esemu SN; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, Cameroon.
Jerome Kfusi A; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, Cameroon.
Ndip RN; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, Cameroon.
Ndip LM; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, Cameroon.; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, United States of America.
Źródło:: PloS one [PLoS One] 2020 Dec 15; Vol. 15 (12), pp. e0235583. Date of Electronic Publication: 2020 Dec 15 (Print Publication: 2020).
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: San Francisco, CA : Public Library of Science
MeSH Terms:: Escherichia coli Infections/*microbiology
Escherichia coli O157/*genetics
Virulence/*genetics
Virulence Factors/*genetics
Animals ; Cameroon ; Cattle ; Escherichia coli Proteins/genetics ; Food Microbiology/methods ; Genetic Profile ; Meat/microbiology ; Polymerase Chain Reaction/methods ; Prevalence
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Substance Nomenclature:: 0 (Escherichia coli Proteins)
0 (Virulence Factors)
Entry Date(s):: Date Created: 20201215 Date Completed: 20210111 Latest Revision: 20210111
Update Code:: 20240104
PubMed Central ID:: PMC7737970
DOI:: 10.1371/journal.pone.0235583
PMID:: 33320853
: Czasopismo naukowe

Pełny tekst

Background: Escherichia coli O157 is an emerging foodborne pathogen of great public health concern. It has been associated with bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome in humans. Most human infections have been traced to cattle and the consumption of contaminated cattle products. In order to understand the risk associated with the consumption of cattle products, this study sought to investigate the prevalence and identify virulence genes in E. coli O157 from cattle in Cameroon.
Method: A total of 512 rectal samples were obtained and analysed using conventional bacteriological methods (enrichment on modified Tryptone Soy Broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar) for the isolation of E. coli O157. Presumptive E. coli O157 isolates were confirmed serologically using E. COLIPROTM O157 latex agglutination test and molecularly using PCR targeting the rfb gene in the isolates. Characterisation of the confirmed E. coli O157 strains was done by amplification of stx1, stx2, eaeA and hlyA virulence genes using both singleplex and multiplex PCR.
Results: E. coli O157 was detected in 56 (10.9%) of the 512 samples examined. The presence of the virulence genes stx2, eaeA and hylA was demonstrated in 96.4% (54/56) of the isolates and stx1 in 40 (71.4%) of the 54. The isolates exhibited three genetic profiles (I-III) with I (stx1, stx2, eaeA and hlyA) being the most prevalent (40/56; 71.4%) while two isolates had none of the virulence genes tested.
Conclusion: A proportion of cattle slaughtered in abattoirs in Buea are infected with pathogenic E. coli O157 and could be a potential source of human infections. We recommend proper animal food processing measures and proper hygiene be prescribed and implemented to reduce the risk of beef contamination.
Competing Interests: The authors have declared that no competing interests exist.

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