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Tytuł:
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SopA inactivation or reduced expression is selected in intracellular Salmonella and contributes to systemic Salmonella infection.
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Autorzy:
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Ma S; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China. Electronic address: .
Liu X; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China. Electronic address: .
Ma S; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China. Electronic address: .
Jiang L; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China. Electronic address: .
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Źródło:
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Research in microbiology [Res Microbiol] 2021 Mar; Vol. 172 (2), pp. 103795. Date of Electronic Publication: 2020 Dec 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Paris : Elsevier
Original Publication: Amsterdam ; New York : Elsevier, [c1989-
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MeSH Terms:
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Bacterial Proteins/*genetics
Bacterial Proteins/*metabolism
Salmonella typhi/*genetics
Salmonella typhimurium/*genetics
Animals ; Cell Line ; Gene Expression Regulation, Bacterial ; Genomic Islands ; HeLa Cells ; Humans ; Macrophages ; Mice ; Mice, Inbred BALB C ; RNA, Untranslated/physiology ; Salmonella Infections/microbiology ; Salmonella typhi/metabolism ; Salmonella typhimurium/metabolism ; U937 Cells
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Contributed Indexing:
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Keywords: IsrM; Negative regulation; S. Paratyphi A; S. Typhi; Salmonella enterica; sopA inactivation
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Substance Nomenclature:
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0 (Bacterial Proteins)
0 (RNA, Untranslated)
EC 3.4.- (SopA protein, Bacteria)
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Entry Date(s):
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Date Created: 20201221 Date Completed: 20210913 Latest Revision: 20210913
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Update Code:
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20240105
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DOI:
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10.1016/j.resmic.2020.103795
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PMID:
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33347947
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Pseudogenes are accumulated in host-restricted Salmonella enterica serovars, while pseudogenization is primarily regarded as a process that purges unnecessary genes from the genome. Here we showed that the inactivation of sopA, which encodes an effector of Salmonella Pathogenicity Island 1, in human-restricted S. enterica serovar Typhi (S. Ty) and Paratyphi A (S. PA) is under positive selection and aimed to reduce bacterial cytotoxicity toward host macrophages. Moreover, we found that the expression of sopA in Salmonella Typhimurium (S. Tm), a broad-host-range serovar which causes systemic disease in mice, was negatively regulated during mice infection and survival in murine macrophages. The sopA repression in S. Tm is mediated by IsrM, a small RNA absent from the genome of S. Ty and S. PA. Due to the lack of IsrM, sopA expression was unregulated in S. Ty and S. PA, which might have facilitated the convergent inactivation of sopA in these two serovars. In conclusion, our findings demonstrate that sopA inactivation or intracellular repression is the target of positive selection during the systemic infection caused by S. enterica serovars.
Competing Interests: Declaration of competing interest There is no conflict of interest.
(Copyright © 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
