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Tytuł:
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Tyrosinase-mediated melanogenesis in melanoma cells: Array comparative genome hybridization integrating proteomics and bioinformatics studies.
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Autorzy:
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Yin SJ; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China.
Lee JR; Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, South Korea.
Hahn MJ; Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, South Korea.
Yang JM; Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul 135-710, South Korea.
Qian GY; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China. Electronic address: .
Park YD; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China; Skin Diseases Research Center, Yangtze Delta Region Institute of Tsinghua University, 705 Yatai Road, Jiaxing 314006, PR China; Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, 705 Yatai Road, Jiaxing 314006, PR China. Electronic address: .
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Źródło:
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International journal of biological macromolecules [Int J Biol Macromol] 2021 Feb 15; Vol. 170, pp. 150-163. Date of Electronic Publication: 2020 Dec 29.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
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MeSH Terms:
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Comparative Genomic Hybridization*
Computational Biology/*methods
Melanins/*biosynthesis
Melanoma/*metabolism
Monophenol Monooxygenase/*metabolism
Neoplasm Proteins/*metabolism
Proteomics/*methods
Cell Line, Tumor ; Chromatography, Liquid ; Chromosomes, Human, Pair 11/genetics ; Clone Cells ; Endoplasmic Reticulum Chaperone BiP ; Gene Dosage ; Gene Ontology ; Humans ; Melanoma/genetics ; Monophenol Monooxygenase/genetics ; Neoplasm Proteins/genetics ; Pigmentation ; Protein Interaction Mapping ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry
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Contributed Indexing:
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Keywords: Array CGH; Melanogenesis; Melanoma
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Substance Nomenclature:
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0 (Endoplasmic Reticulum Chaperone BiP)
0 (HSPA5 protein, human)
0 (Melanins)
0 (Neoplasm Proteins)
EC 1.14.18.1 (Monophenol Monooxygenase)
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Entry Date(s):
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Date Created: 20201228 Date Completed: 20210414 Latest Revision: 20211204
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Update Code:
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20240105
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DOI:
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10.1016/j.ijbiomac.2020.12.146
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PMID:
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33359255
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We investigated the tyrosinase-associated melanogenesis in melanoma cells by using OMICS techniques. We characterized the chromosome copy numbers, including Chr 11q21 where the tyrosinase gene is located, from several melanoma cell lines (TXM13, G361, and SK-MEL-28) by using array CGH. We revealed that 11q21 is stable in TXM13 cells, which is directly related to a spontaneous high melanin pigment production. Meanwhile, significant loss of copy number of 11q21 was found in G361 and SK-MEL-28. We further profiled the proteome of TXM13 cells by LC-ESI-MSMS and detected more than 900 proteins, then predicted 11 hub proteins (YWHAZ; HSP90AA1; HSPA5; HSPA1L; HSPA9; HSP90B1; HSPA1A; HSPA8; FKSG30; ACTB; DKFZp686DQ972) by using an interactomic algorithm. YWHAZ (25% interaction in the network) is thought to be a most important protein as a linking factor between tyrosinase-triggered melanogenesis and melanoma growth. Bioinformatic tools were further applied for revealing various physiologic mechanisms and functional classification. The results revealed clues for the spontaneous pigmentation capability of TXM13 cells, contrary to G361 and SK-MEL-28 cells, which commonly have depigmentation properties during subculture. Our study comparatively conducted the genome-wide screening and proteomic profiling integrated interactomics prediction for TXM13 cells and suggests new insights for studying both melanogenesis and melanoma.
Competing Interests: Declaration of competing interest None.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
