Autolysis of Chlorella variabilis in Starving Paramecium bursaria Help the Host Cell Survive Against Starvation Stress.

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Tytuł:: Autolysis of Chlorella variabilis in Starving Paramecium bursaria Help the Host Cell Survive Against Starvation Stress.
Autorzy:: Kodama Y; Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue, Shimane, Japan. .
Miyazaki S; Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan.
Źródło:: Current microbiology [Curr Microbiol] 2021 Feb; Vol. 78 (2), pp. 558-565. Date of Electronic Publication: 2021 Jan 03.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: New York, Springer International.
MeSH Terms:: Chlorella*
Paramecium*
Darkness ; Microscopy, Fluorescence ; Symbiosis
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Grant Information:: 17K07513 Japan Society for the Promotion of Science
Contributed Indexing:: Keywords: Algae; Ciliate; Endosymbiosis; Indirect immunofluorescence microscopy; Monoclonal antibody; Protist
Entry Date(s):: Date Created: 20210103 Date Completed: 20210514 Latest Revision: 20210514
Update Code:: 20240105
DOI:: 10.1007/s00284-020-02304-9
PMID:: 33389061
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The endosymbiosis between Paramecium bursaria and Chlorella spp. is mutualistic. Symbiotic algae localize beneath the host Paramecium cell cortex compete for their attachment sites with preexisting organelle trichocysts. To examine the relationship between P. bursaria trichocysts and their symbiotic algae, algae-bearing or alga-free P. bursaria were starved for several days and the changes in the number of Chlorella sp. and presence or absence of trichocysts were evaluated. We conducted an indirect immunofluorescence microscopy with an anti-trichocyst monoclonal antibody against P. bursaria cells. Indirect immunofluorescence microscopy demonstrated that under starvation and darkness conditions, the immunofluorescence of trichocysts in alga-free P. bursaria decreased much faster than that in the normal algae-bearing P. bursaria. In the latter case, our observations proposed the possibility that the nutrition obtained from symbiotic algal digestion may promote trichocysts synthesis. This algal digestion mechanism may permit host P. bursaria cells to survive for a longer time under starvation condition. To the best of our knowledge, this may be a new benefit that host P. bursaria gain from harboring symbiotic algae.

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