Proteomic analysis from skin swabs reveals a new set of proteins identifying skin impairment in atopic dermatitis.

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Abstrakt

Tytuł:: Proteomic analysis from skin swabs reveals a new set of proteins identifying skin impairment in atopic dermatitis.
Autorzy:: Morelli P; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Gaspari M; Department of Experimental and Clinical Medicine, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Gabriele C; Department of Experimental and Clinical Medicine, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Dastoli S; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Bennardo L; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Pavel AB; Department of Biomedical Engineering, University of Mississippi, University, MS, USA.
Patruno C; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Del Duca E; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Nisticò SP; Department of Health Science, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy.
Źródło:: Experimental dermatology [Exp Dermatol] 2021 Jun; Vol. 30 (6), pp. 811-819. Date of Electronic Publication: 2021 Jan 23.
Typ publikacji:: Comparative Study; Journal Article
Język:: English
Imprint Name(s):: Original Publication: Copenhagen : Munksgaard, c1992-
MeSH Terms:: Dermatitis, Atopic/*metabolism
Adult ; Female ; Humans ; Male ; Middle Aged ; Proteomics ; Young Adult
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Contributed Indexing:: Keywords: TDRD15; atopic dermatitis; barrier; proteomic; skin swab
Entry Date(s):: Date Created: 20210104 Date Completed: 20220322 Latest Revision: 20220322
Update Code:: 20240105
DOI:: 10.1111/exd.14276
PMID:: 33394542
: Czasopismo naukowe

Pełny tekst

Atopic Dermatitis (AD) is a common inflammatory skin disease characterized by skin and systemic inflammation, and barrier dysfunction. Herein, we investigate the proteomic profile of AD skin barrier to identify a unique signature with an easy-performed sampling approach. We enrolled 8 moderate-to-severe AD patients and 8 age- and gender-matched healthy controls. Swabs were obtained from non-lesional skin of retroauricular area and antecubital fold. Peptide mixtures obtained through protein precipitation and in-solution digestion were analysed using NanoLC-MS/MS. Label-free quantification and statistical analysis were conducted in MaxQuant and Perseus. Bioinformatics analysis was performed using Gene Ontology and STRING. We identified 908 proteins and 35 differentially expressed proteins were selected (fold change 2, FDR < 0.05). Particularly, AD skin showed downregulation of skin hydration factors, structural and epidermal proteins, abnormalities in protease-proteasome complex and lipid metabolism profile. Imbalance of antioxidant and inflammatory processes, along with TDRD15 upregulation was also observed. Our result showed partial overlap with skin biopsy/tape-strips studies, showing the reliability of our sampling approach which could be an easier method of detection of hallmark barrier proteins in AD. Furthermore, we displayed a new differentially expressed set of proteins, not yet explored in AD which can have a potential role in AD pathomechanisms.
(© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

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