In Vivo Evaluation of Combined CK2 Inhibition and Irradiation in Human WiDr Tumours.

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Abstrakt

Tytuł:: In Vivo Evaluation of Combined CK2 Inhibition and Irradiation in Human WiDr Tumours.
Autorzy:: Zwicker F; Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany; .; Clinical Cooperation Unit Molecular Radiation Oncology, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
Hauswald H; Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.
Weber KJ; Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.
Debus J; Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.; Clinical Cooperation Unit Molecular Radiation Oncology, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
Huber PE; Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.; Clinical Cooperation Unit Molecular Radiation Oncology, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
Źródło:: In vivo (Athens, Greece) [In Vivo] 2021 Jan-Feb; Vol. 35 (1), pp. 111-117.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: <2000- > : Attiki, Greece : International Institute of Anticancer Research
Original Publication: Athens : Dr. J.G. Delinassios
MeSH Terms:: Casein Kinase II*/genetics
Neoplasms*
Animals ; Apoptosis ; Cell Line, Tumor ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude
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Contributed Indexing:: Keywords: CK2 inhibition; TBB; human WiDr tumours; irradiation; tetra-bromobenzotriazole
Substance Nomenclature:: EC 2.7.11.1 (Casein Kinase II)
Entry Date(s):: Date Created: 20210106 Date Completed: 20210618 Latest Revision: 20210618
Update Code:: 20240105
PubMed Central ID:: PMC7880802
DOI:: 10.21873/invivo.12238
PMID:: 33402456
: Czasopismo naukowe

Background/aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in mammalian cells in vitro. Here, we investigated potential radiosensitization by CK2 inhibition in vivo.
Materials and Methods: The effect of CK2 inhibition in vivo was investigated in human WiDr-xenograft tumours grown subcutaneously on BALB/c nu/nu mice with and without fractionated irradiation. CK2 inhibition was performed using the specific inhibitor tetra-bromobenzotriazole (TBB). Histological examinations included staining for apoptosis and double-strand breaks.
Results: Both TBB treatment alone and radiation alone significantly reduced tumour growth, which was reflected by increased apoptosis rates. However, TBB treatment did not boost radiation-induced tumour growth suppression in combined treatment, although the apoptosis rate increased and repair of double-strand breaks was reduced. This was in stark contrast to previous data on in vitro radiosensitization.
Conclusion: The absence of radiosensitization by CK2 inhibition should be investigated in different tumour models.
(Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)

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