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Tytuł:
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Ibandronate-Loaded Carbon Nanohorns Fabricated Using Calcium Phosphates as Mediators and Their Effects on Macrophages and Osteoclasts.
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Autorzy:
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Nakamura M; Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Ueda K; Biomedical Engineering Division, Graduate School of Medicine, Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Yamamoto Y; Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Aoki K; Physical Therapy Division, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Zhang M; CNT Application Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Saito N; Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Yudasaka M; Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.; Faculty of Science & Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan.
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Źródło:
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ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Jan 27; Vol. 13 (3), pp. 3701-3712. Date of Electronic Publication: 2021 Jan 06.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, D.C. : American Chemical Society
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MeSH Terms:
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Bone Density Conservation Agents/*pharmacology
Drug Carriers/*chemistry
Ibandronic Acid/*pharmacology
Macrophages/*drug effects
Nanotubes, Carbon/*chemistry
Osteoclasts/*drug effects
Animals ; Bone Density Conservation Agents/administration & dosage ; Calcium Phosphates/chemistry ; Ibandronic Acid/administration & dosage ; Mice ; Nanotubes, Carbon/ultrastructure ; RAW 264.7 Cells
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Contributed Indexing:
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Keywords: calcium phosphate; carbon nanohorns; ibandronate; macrophage; osteoclast
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Substance Nomenclature:
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0 (Bone Density Conservation Agents)
0 (Calcium Phosphates)
0 (Drug Carriers)
0 (Nanotubes, Carbon)
UMD7G2653W (Ibandronic Acid)
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Entry Date(s):
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Date Created: 20210107 Date Completed: 20210302 Latest Revision: 20210302
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Update Code:
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20240105
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DOI:
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10.1021/acsami.0c20923
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PMID:
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33406818
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Carbon nanohorns (CNHs), a type of nanocarbon, have been studied for the application of drug delivery systems (DDSs) because they are easily functionalized, support bone regeneration, can be used to perform photohyperthermia, have low toxicity, and are easily phagocytosed by macrophages. To take advantage of these features of CNHs, we developed a DDS for the local treatment of bone metastasis by loading the antibone resorption drug ibandronate (IBN) onto CNHs. The poor adsorption of IBN onto CNHs due to the weak hydrophilic-hydrophobic interaction was overcome by using calcium phosphates (CaPs) as mediators. In the fabrication process, we used oxidized CNH (OxCNH), which is less hydrophobic, onto which IBN was coprecipitated with CaP from a labile supersaturated CaP solution. OxCNH-CaP-IBN composite nanoparticles exerted stronger cell-suppressive effects than OxCNH and IBN in both murine macrophages (RAW264.7 cells) and osteoclasts (differentiated from RAW264.7 cells). OxCNH-CaP-IBN composite nanoparticles were efficiently phagocytosed by macrophage cells, where they specifically accumulated in lysosomes. The stronger cell-suppressive effects were likely due to intracellular delivery of IBN, i.e., the release of IBN from OxCNH-CaP-IBN composite nanoparticles via dissociation of CaP in the acidic environment of lysosomes. Our findings suggest that OxCNH-CaP-IBN composite nanoparticles are potentially useful for the local treatment of metastatic bone destruction.
