The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.

Tytuł:: The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.
Autorzy:: Sloan MA; Department of Biochemistry, University of Oxford, South Parks Rd, Oxford, OX1 3QU, UK.
Sadlova J; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Lestinova T; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Sanders MJ; The Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, Cambridgeshire, UK.
Cotton JA; The Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, Cambridgeshire, UK.
Volf P; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Ligoxygakis P; Department of Biochemistry, University of Oxford, South Parks Rd, Oxford, OX1 3QU, UK. .
Źródło:: Parasites & vectors [Parasit Vectors] 2021 Jan 06; Vol. 14 (1), pp. 15. Date of Electronic Publication: 2021 Jan 06.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central
MeSH Terms:: Gene Expression Profiling*
Trypanosomatina*
Phlebotomus/*parasitology
Animals ; Blood/parasitology ; Feeding Behavior ; Humans ; Insect Vectors/metabolism ; Insect Vectors/parasitology ; Leishmania infantum ; Leishmania major ; Leishmaniasis/parasitology ; Leishmaniasis/transmission ; Phlebotomus/metabolism
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Grant Information:: United Kingdom WT_ Wellcome Trust; BB/K003569 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 31012 H2020 European Research Council
Entry Date(s):: Date Created: 20210107 Date Completed: 20210511 Latest Revision: 20211005
Update Code:: 20240105
PubMed Central ID:: PMC7789365
DOI:: 10.1186/s13071-020-04498-0
PMID:: 33407867
: Czasopismo naukowe

Pełny tekst

Background: Leishmaniasis, caused by parasites of the genus Leishmania, is a disease that affects up to 8 million people worldwide. Parasites are transmitted to human and animal hosts through the bite of an infected sand fly. Novel strategies for disease control require a better understanding of the key step for transmission, namely the establishment of infection inside the fly.
Methods: The aim of this work was to identify sand fly systemic transcriptomic signatures associated with Leishmania infection. We used next generation sequencing to describe the transcriptome of whole Phlebotomus papatasi sand flies when fed with blood alone (control) or with blood containing one of three trypanosomatids: Leishmania major, L. donovani and Herpetomonas muscarum, the latter being a parasite not transmitted to humans.
Results: Of the trypanosomatids studied, only L. major was able to successfully establish an infection in the host P. papatasi. However, the transcriptional signatures observed after each parasite-contaminated blood meal were not specific to success or failure of a specific infection and they did not differ from each other. The transcriptional signatures were also indistinguishable after a non-contaminated blood meal.
Conclusions: The results imply that sand flies perceive Leishmania as just one feature of their microbiome landscape and that any strategy to tackle transmission should focus on the response towards the blood meal rather than parasite establishment. Alternatively, Leishmania could suppress host responses. These results will generate new thinking around the concept of stopping transmission by controlling the parasite inside the insect.

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