Protection studies of an excretory-secretory protein HcABHD against Haemonchus contortus infection.

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Abstrakt

Tytuł:: Protection studies of an excretory-secretory protein HcABHD against Haemonchus contortus infection.
Autorzy:: Lu M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Tian X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Zhang Y; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Wang W; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Tian AL; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, P. R. China.
Aimulajiang K; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Liu L; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Li C; Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, 20705, USA.
Yan R; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Xu L; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Song X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China.
Li X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, P. R. China. .
Źródło:: Veterinary research [Vet Res] 2021 Jan 06; Vol. 52 (1), pp. 3. Date of Electronic Publication: 2021 Jan 06.
Typ publikacji:: Journal Article; Randomized Controlled Trial, Veterinary
Język:: English
Imprint Name(s):: Publication: 2011- : London : BioMed Central
Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-
MeSH Terms:: Goat Diseases/*parasitology
Haemonchiasis/*veterinary
Haemonchus/*immunology
Helminth Proteins/*therapeutic use
Vaccines/*therapeutic use
Animals ; Antigens, Helminth/therapeutic use ; Enzyme-Linked Immunosorbent Assay ; Female ; Goat Diseases/prevention & control ; Goats ; Haemonchiasis/prevention & control ; Male ; Parasite Egg Count/veterinary ; Vaccines, Synthetic/therapeutic use
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Grant Information:: 2017YFD0501200 National Key Research and Development Program of China; BZ2019013 Policy Guidance Project of Jiangsu Province for International Scientific and Technological Cooperation; 2015CB150300 National Key Basic Research Program (973 Program)
Contributed Indexing:: Keywords: H. contortus; excretory–secretory protein; goats; immunization; Α/β-hydrolase
Substance Nomenclature:: 0 (Antigens, Helminth)
0 (Helminth Proteins)
0 (Vaccines)
0 (Vaccines, Synthetic)
Entry Date(s):: Date Created: 20210107 Date Completed: 20210121 Latest Revision: 20210121
Update Code:: 20240105
PubMed Central ID:: PMC7786147
DOI:: 10.1186/s13567-020-00871-0
PMID:: 33407892
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Unlike the successful immunization of native H. contortus antigens that contributed to the realization of the first commercial vaccine Barbervax, not many studies revealed the encouraging protective efficacies of recombinant H. contortus antigens in laboratory trials or under field conditions. In our preliminary study, H. contortus α/β-hydrolase domain protein (HcABHD) was demonstrated to be an immunomodulatory excretory-secretory (ES) protein that interacts with goat T cells. We herein evaluated the protective capacities of two HcABHD preparations, recombinant HcABHD (rHcABHD) antigen and anti-rHcABHD IgG, against H. contortus challenge via active and passive immunization trials, respectively. Parasitological parameter, antibody responses, hematological pathology and cytokine profiling in unchallenged and challenged goats were monitored and determined throughout both trials. Subcutaneous administration of rHcABHD with Freund adjuvants elicited protective immune responses in challenged goats, diminishing cumulative fecal egg counts (FEC) and total worm burden by 54.0% and 74.2%, respectively, whereas passive immunization with anti-rHcABHD IgG conferred substantial protection to challenged goats by generating a 51.5% reduction of cumulative FEC and a 73.8% reduction of total worm burden. Additionally, comparable changes of mucosal IgA levels, circulating IgG levels, hemoglobin levels, and serum interleukin (IL)-4 and IL-17A levels were observed in rHcABHD protein/anti-rHcABHD IgG immunized goats in both trials. Taken together, the recombinant version of HcABHD might have further application under field conditions in protecting goats against H. contortus infection, and the integrated immunological pipeline of ES antigen identification, screening and characterization may provide new clues for further development of recombinant subunit vaccines to control H. contortus.

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