[Development and maintenance of the neuromuscular system].

Szczegóły
Abstrakt

Tytuł:: [Development and maintenance of the neuromuscular system].
Autorzy:: Guillermin C; Institut De Génomique Fonctionnelle De Lyon, ENS de Lyon, UMR CNRS 5242, Lyon, France.
Bouchet M; Institut De Génomique Fonctionnelle De Lyon, ENS de Lyon, UMR CNRS 5242, Lyon, France.
Garcès A; Institut De Génomique Fonctionnelle De Lyon, ENS de Lyon, UMR CNRS 5242, Lyon, France.
Enriquez J; Institut De Génomique Fonctionnelle De Lyon, ENS de Lyon, UMR CNRS 5242, Lyon, France.
Transliterated Title:: Développement et maintenance du système neuromusculaire.
Źródło:: Medecine sciences : M/S [Med Sci (Paris)] 2020 Dec; Vol. 36 Hors série n° 2, pp. 13-16. Date of Electronic Publication: 2021 Jan 11.
Typ publikacji:: Journal Article
Język:: French
Imprint Name(s):: Publication: <2003->: Paris : EDK
Original Publication: [Paris] : Flammarion, [1985-
MeSH Terms:: Muscle, Skeletal/*growth & development
Muscle, Skeletal/*physiology
Regeneration/*physiology
Adult ; Animals ; Axons/physiology ; CRISPR-Cas Systems ; Gene Expression Regulation, Developmental ; Genomics/methods ; Humans ; Motor Neurons/physiology ; Muscle, Skeletal/innervation ; RNA-Seq ; Regeneration/genetics
References:: Catela C , Shin MM , Dasen JS . Assembly and function of spinal circuits for motor control. Annu Rev Cell Dev Biol 2015 ; 31 : 669–698.
Ruiz-Cañada C , Budnik V . Introduction on the use of the Drosophila embryonic/larval neuromuscular junction as a model system to study synapse development and function, and a brief summary of pathfinding and target recognition. Int Rev Neurobiol 2006 ; 75 : 1–31.
Soler C , Daczewska M , Da Ponte JP , et al. Coordinated development of muscles and tendons of the Drosophila leg. Development 2004 ; 131 : 6041–6051.
Venkatasubramanian L , Guo Z , Xu S , et al. Stereotyped terminal axon branching of leg motor neurons mediated by IgSF proteins DIP-a and Dpr10. Elife 2019 ; 8 : e42692.
Worden MK , Hwang JC , Vélez SJ , et al. Regeneration studies on a crayfish neuromuscular system. I. Connectivity changes after intersegmental nerve transplants. J Neurobiol 1988 ; 19 : 127–140.
Huebner EA , Strittmatter SM . Axon regeneration in the peripheral and central nervous systems. Results Probl Cell Differ 2009 ; 48 : 339–351.
Enriquez J , Venkatasubramanian L , Baek M , et al. Specification of individual adult motor neuron morphologies by combinatorial transcription factor codes. Neuron 2015 ; 86 : 955–970.
Enriquez J , Rio LQ , Blazeski R , et al. Differing strategies despite shared lineages of motor neurons and glia to achieve robust development of an adult neuropil in Drosophila. Neuron 2018 ; 97 : 538–554.
Baek M , Mann RS . Lineage and birth date specify motor neuron targeting and dendritic architecture in adult Drosophila. J Neurosci 2009 ; 29 : 6904–6916.
Heiman M , Schaefer A , Gong S , et al. A translational profiling approach for the molecular characterization of CNS cell types. Cell 2008 ; 135 : 738–748.
Entry Date(s):: Date Created: 20210111 Date Completed: 20211029 Latest Revision: 20211029
Update Code:: 20240105
DOI:: 10.1051/medsci/2020207
PMID:: 33427630
: Czasopismo naukowe

Diseases of the locomotor system are at the origin of disabilities with severe social and economic consequences. The study of the neuromuscular system development and maintenance has become a key challenge for the scientific community in order to design efficient therapies. My thesis project aims to elucidate the mechanisms at the origin of the communication between motoneuron axons and their muscle targets in order to understand how specific innervations are generated during development and maintained during adulthood. The first part of the project will address the understanding of the mechanisms controlling the specific muscle-axon recognition during development. I will perform live imaging and fixed tissues experiments to visualize and understand the development of myoblasts and motoneurons at the same time. Then, I will perform transcriptomic experiments to discover molecules playing a role in the specific axon-muscle recognition. The second part of the project is meant to elucidate the mechanism controlling the system maintenance in the adult. To answer this question I will study the function of morphological transcription factors in adulthood, which are known as transcription factors controlling the morphology of motoneurons during development. To conclude, this project will lead to novel biological concepts that will increase our fundamental knowledge on developmental biology. Understanding the mechanisms that specify the muscle innervation will allow to find efficient ways to tackle neuromuscular diseases.
(© 2020 médecine/sciences – Inserm.)

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