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Tytuł:
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Synthesis and chromatographic evaluation of pyrazinedicarboxylic anhydride bonded stationary phase.
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Autorzy:
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Jin G; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China.
Ding J; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Zhou Y; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China.
Xia D; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China.
Guo Z; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, China. Electronic address: .
Liang X; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, China.
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Źródło:
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Journal of chromatography. A [J Chromatogr A] 2021 Feb 08; Vol. 1638, pp. 461825. Date of Electronic Publication: 2021 Jan 06.
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Typ publikacji:
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Evaluation Study; Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam ; New York : Elsevier, 1993-
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MeSH Terms:
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Anhydrides/*chemistry
Chromatography/*methods
Dicarboxylic Acids/*chemistry
Pyrazines/*chemistry
Alkaloids/isolation & purification ; Dicarboxylic Acids/chemical synthesis ; Flavonoids/isolation & purification ; Hydrophobic and Hydrophilic Interactions ; Magnetic Resonance Spectroscopy ; Oligosaccharides/chemistry ; Pyrazines/chemical synthesis ; Silicon Dioxide/chemistry ; Water/chemistry
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Contributed Indexing:
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Keywords: Amino/anhydride bonded; Hydrophilic interaction liquid chromatography; Selectivity; Stationary phase; TCMs
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Substance Nomenclature:
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0 (Alkaloids)
0 (Anhydrides)
0 (Dicarboxylic Acids)
0 (Flavonoids)
0 (Oligosaccharides)
0 (Pyrazines)
059QF0KO0R (Water)
7631-86-9 (Silicon Dioxide)
QNN35WOF29 (2,3-pyrazinedicarboxylic acid)
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Entry Date(s):
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Date Created: 20210115 Date Completed: 20210219 Latest Revision: 20210219
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Update Code:
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20240105
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DOI:
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10.1016/j.chroma.2020.461825
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PMID:
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33450715
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A silica based hydrophilic stationary phase bonding with 2,3-pyrazinedicarboxylic anhydride and amino groups was synthesized via amino-acid anhydride ring opening reaction. The bonded groups could not only provide hydrophilic interaction, but also electrostatic, π-π and hydrogen bonding interactions, etc. The results of characterization with elemental analysis and solid-state 13 C cross-polarization magic-angle-spinning NMR indicated the successful preparation of amino and carboxyl bonded stationary phase named ZAC. The ζ-potential of ZAC stationary phase showed the negatively charge was dominate at pH larger than 3.5. Chromatographic evaluation revealed that ZAC stationary phase behaved well under HILIC mode. It showed different selectivity and retention compared to some typical commercial columns, and it was validated by the separation of chitooligosaccharides, flavonoid glycosides, organic acids and alkaloid samples. Based on the different selectivity between ZAC stationary phase and C18 columns, ZAC stationary phase also showed different selectivity with C18. And it was verified by the separation of Lonicerae Japonicae Flos and Menispermi Rhizoma extracts.
Competing Interests: Declaration of Competing Interest The authors have declared no conflict of interest.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
