An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca reveals new insights into ion signaling in plants.
Li K; Department of Botany I, Julius-Von-Sachs Institute for Biosciences, University of Wuerzburg, Wuerzburg, 97082, Germany.
Prada J; Department of Bioinformatics, University of Wuerzburg, Wuerzburg, 97074, Germany.
Damineli DSC; Department of Cell Biology & Molecular Genetics, University of Maryland, 2136 Bioscience Research Bldg, College Park, MD, 20742-5815, USA.; Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, 01246-903, Brazil.
Liese A; Leibniz Institute of Plant Biochemistry, Halle (Saale), 06120, Germany.
Romeis T; Leibniz Institute of Plant Biochemistry, Halle (Saale), 06120, Germany.
Dandekar T; Department of Bioinformatics, University of Wuerzburg, Wuerzburg, 97074, Germany.
Feijó JA; Department of Cell Biology & Molecular Genetics, University of Maryland, 2136 Bioscience Research Bldg, College Park, MD, 20742-5815, USA.
Hedrich R; Department of Botany I, Julius-Von-Sachs Institute for Biosciences, University of Wuerzburg, Wuerzburg, 97082, Germany.
Konrad KR; Department of Botany I, Julius-Von-Sachs Institute for Biosciences, University of Wuerzburg, Wuerzburg, 97082, Germany.
The New phytologist [New Phytol] 2021 Jan 17. Date of Electronic Publication: 2021 Jan 17.
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Grant Information :
19/23343-7 Fundação de Amparo à Pesquisa do Estado de São Paulo; KO3657/2-3 Deutsche Forschungsgemeinschaft; NO. 201706320320 Chinese scholarship council; R01 GM131043 United States GM NIGMS NIH HHS; NSF 709 (MCB1616437, MCB1637673, and MCB1930165) Foundation for the National Institutes of Health; MCB1616437 National Science Foundation; MCB1637673 National Science Foundation; MCB1930165 National Science Foundation
Contributed Indexing :
Keywords: abscisic acid (ABA); calcium; flg22; guard cells; imaging; ion signaling; pH; pollen tube
Entry Date(s) :
Date Created: 20210117 Latest Revision: 20210218
Update Code :
Whereas the role of calcium ions (Ca 2+ ) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca 2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca 2+ - and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca 2+ -dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca 2+ . In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca 2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca 2+ -increases and cytosolic acidification by c. 2 min, suggesting a Ca 2+ /pH-independent early pathogen signaling step. Imaging Ca 2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca 2+ - and pH-responses. We propose close interrelation in Ca 2+ - and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.
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