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Tytuł pozycji:

Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.

Tytuł :
Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
Autorzy :
Korotkyi O; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Huet A; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Dvorshchenko K; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Kobyliak N; Bogomolets National Medical University, Kyiv, Ukraine. .; Laboratory of Pathology CSD, Kyiv, Ukraine. .
Falalyeyeva T; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Ostapchenko L; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Pokaż więcej
Źródło :
Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2021 Jan 18. Date of Electronic Publication: 2021 Jan 18.
Publication Model :
Ahead of Print
Typ publikacji :
Journal Article
Język :
Imprint Name(s) :
Original Publication: New York, NY. : Springer
References :
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Contributed Indexing :
Keywords: Biochemical markers of cartilage metabolism; Chondroprotector; Cytokines; Dysbiosis; Inflammation; Toll-like receptors
Entry Date(s) :
Date Created: 20210118 Latest Revision: 20210118
Update Code :
Czasopismo naukowe
The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken. The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats. Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR. The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay. Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage. MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased. Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage. The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1). The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control. The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.

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