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Tytuł:
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A review of Cryptosporidium spp. and their detection in water.
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Autorzy:
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Hassan EM; Department of Civil and Environmental Engineering, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, Canada E-mail: .
Örmeci B; Department of Civil and Environmental Engineering, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, Canada E-mail: .
DeRosa MC; Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Canada, K1S 5B6.
Dixon BR; Bureau of Microbial Hazards, Food Directorate, Health Canada, Ottawa, Canada, K1A 0K9.
Sattar SA; Department of Civil and Environmental Engineering, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, Canada E-mail: ; C.R.E.M. Co Labs, Units 1-2, 3403 American Drive, Mississauga, ON, Canada, L4V 1T4.
Iqbal A; C.R.E.M. Co Labs, Units 1-2, 3403 American Drive, Mississauga, ON, Canada, L4V 1T4.
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Źródło:
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Water science and technology : a journal of the International Association on Water Pollution Research [Water Sci Technol] 2021 Jan; Vol. 83 (1), pp. 1-25.
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Typ publikacji:
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Journal Article; Review
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Język:
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English
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Imprint Name(s):
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Publication: <1998->: London : IWA Publishing
Original Publication: Oxford ; New York : Pergamon Press, 1981-
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MeSH Terms:
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Cryptosporidiosis*
Cryptosporidium*/genetics
Cryptosporidium parvum*
Animals ; Oocysts ; Water
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Substance Nomenclature:
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059QF0KO0R (Water)
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Entry Date(s):
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Date Created: 20210118 Date Completed: 20210120 Latest Revision: 20210120
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Update Code:
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20240105
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PubMed Central ID:
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wst_2020_515
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DOI:
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10.2166/wst.2020.515
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PMID:
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33460403
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Cryptosporidium spp. are one of the most important waterborne pathogens worldwide and a leading cause of mortality from waterborne gastrointestinal diseases. Detection of Cryptosporidium spp. in water can be very challenging due to their low numbers and the complexity of the water matrix. This review describes the biology of Cryptosporidium spp. and current methods used in their detection with a focus on C. parvum and C. hominis. Among the methods discussed and compared are microscopy, immunology-based methods using monoclonal antibodies, molecular methods including PCR (polymerase chain reaction)-based assays, and emerging aptamer-based methods. These methods have different capabilities and limitations, but one common challenge is the need for better sensitivity and specificity, particularly in the presence of contaminants. The application of DNA aptamers in the detection of Cryptosporidium spp. oocysts shows promise in overcoming these challenges, and there will likely be significant developments in aptamer-based sensors in the near future.
