-
Tytuł:
-
Development and Testing of a Continuous Flow-Electrical-Split-Flow Lateral Transport Thin Separation System (Fl-El-SPLITT).
-
Autorzy:
-
Shiri F; Department of Mechanical Engineering, University of Utah, 1495 E 100 S, Salt Lake City, Utah 84112, United States.
Gale BK; Department of Mechanical Engineering, University of Utah, 1495 E 100 S, Salt Lake City, Utah 84112, United States.
Sant H; Department of Mechanical Engineering, University of Utah, 1495 E 100 S, Salt Lake City, Utah 84112, United States.
King B; Department of Biomedical Engineering, University of Utah, 36 S. Wasatch Drive, Rm. 3100, Salt Lake City, Utah 84112, United States.
Bardi GT; Department of Pharmacology and Toxicology & James Graham Brown Cancer Center & Hepatobiology and Toxicology COBRE, Kosair Charities Clinical & Translational Research Building, University of Louisville, Room 308, 505 South Hancock Street, Louisville, Kentucky 40202, United States.
Hood JL; Department of Pharmacology and Toxicology & James Graham Brown Cancer Center & Hepatobiology and Toxicology COBRE, Kosair Charities Clinical & Translational Research Building, University of Louisville, Room 308, 505 South Hancock Street, Louisville, Kentucky 40202, United States.
Petersen KE; Department of Mechanical Engineering, University of Utah, 1495 E 100 S, Salt Lake City, Utah 84112, United States.
-
Źródło:
-
Analytical chemistry [Anal Chem] 2021 Feb 09; Vol. 93 (5), pp. 2888-2897. Date of Electronic Publication: 2021 Jan 21.
-
Typ publikacji:
-
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Washington, American Chemical Society.
-
MeSH Terms:
-
Chemical Fractionation*
Electricity*
Particle Size ; Physical Phenomena
-
Grant Information:
-
R21 GM107894 United States GM NIGMS NIH HHS; HHSN261201600054C United States CA NCI NIH HHS; P20 GM113226 United States GM NIGMS NIH HHS
-
Entry Date(s):
-
Date Created: 20210121 Date Completed: 20210621 Latest Revision: 20210621
-
Update Code:
-
20240105
-
DOI:
-
10.1021/acs.analchem.0c04345
-
PMID:
-
33476126
-
In this work, a new high-volume, continuous particle separation device that separates based upon size and charge is described. Two continuous flow-electrical-split-flow lateral transport thin (Fl-El-SPLITT) device architectures (a platinum electrode on a porous membrane and a porous graphite electrode under a membrane) were developed and shown to improve particle separations over a purely electrical-SPLITT device. The graphite FL-El-SPLITT device architecture achieved the best separation of approximately 60% of small (28 nm) vs large (1000 nm) polystyrene particles. Fl-El-SPLITT (platinum) achieved a 75% separation on a single pass using these same particles. Fl-El-SPLITT (platinum) achieved a moderate 26% continuous separation of U87 glioma cell-derived small extracellular vesicles (EVs) from medium EVs. Control parameter testing showed that El-SPLITT continuously directed particle motility within a channel to exit a selected port based upon the applied voltage using either direct current or alternating current. The transition from one port to the other was dependent upon the voltage applied. Both large and small polystyrene particles transitioned together rather than separating at each of the applied voltages. These data present the first ever validation of El-SPLITT in continuous versus batch format. The Fl-El-SPLITT device architecture, monitoring, and electrical and fluid interfacing systems are described in detail for the first time. Capabilities afforded to the system by the flow addition include enhanced particle separation as well as the ability to filter out small particles or desalinate fluids. High-throughput continuous separations based upon electrophoretic mobility will be streamlined by this new technique that combines electrical and flow fields into a single device.
