Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells.

Tytuł:
New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells.
Autorzy:
Chesneau M; CHU Nantes, Université de Nantes, Inserm, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, Nantes, France.; Labex IGO, Nantes, France.
Le Mai H; CHU Nantes, Université de Nantes, Inserm, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, Nantes, France.; Labex IGO, Nantes, France.
Brouard S; CHU Nantes, Université de Nantes, Inserm, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, Nantes, France. .; Labex IGO, Nantes, France. .; Centre d'Investigation Clinique en Biothérapie, Centre de ressources biologiques (CRB), Nantes, France. .
Źródło:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2270, pp. 203-216.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:
B-Lymphocytes, Regulatory/*immunology
Cell Culture Techniques/*methods
Granzymes/*isolation & purification
Apoptosis/immunology ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; B-Lymphocytes, Regulatory/metabolism ; Cell Proliferation ; Granzymes/immunology ; Granzymes/metabolism ; Humans ; Lymphocyte Activation ; T-Lymphocytes, Cytotoxic/immunology
References:
Parker Harp CR, Archambault AS, Sim J, Ferris ST, Mikesell RJ, Koni PA, Shimoda M, Linington C, Russell JH, Wu GF (2015) B cell antigen presentation is sufficient to drive neuroinflammation in an animal model of multiple sclerosis. J Immunol 194:5077–5084. https://doi.org/10.4049/jimmunol.1402236. (PMID: 10.4049/jimmunol.1402236258955314433779)
Liu S (2018) Release of antibodies and cytokines from B cells. Methods Mol Biol 1868:201–207. https://doi.org/10.1007/978-1-4939-8802-0_20. (PMID: 10.1007/978-1-4939-8802-0_2030244467)
Matsushita T (2019) Regulatory and effector B cells: friends or foes? J Dermatol Sci 93:2–7. https://doi.org/10.1016/j.jdermsci.2018.11.008. (PMID: 10.1016/j.jdermsci.2018.11.00830514664)
Floudas A, Amu S, Fallon PG (2016) New insights into IL-10 dependent and IL-10 independent mechanisms of regulatory B cell immune suppression. J Clin Immunol 36(Suppl 1):25–33. https://doi.org/10.1007/s10875-016-0263-8. (PMID: 10.1007/s10875-016-0263-827008462)
Wąsik M, Nazimek K, Bryniarski K (2018) Regulatory B cell phenotype and mechanism of action: the impact of stimulating conditions. Microbiol Immunol 62:485–496. https://doi.org/10.1111/1348-0421.12636. (PMID: 10.1111/1348-0421.1263629998521)
Hagn M, Ebel V, Sontheimer K, Schwesinger E, Lunov O, Beyer T, Fabricius D, Barth TFE, Viardot A, Stilgenbauer S, Hepp J, Scharffetter-Kochanek K, Simmet T, Jahrsdörfer B (2010) CD5+ B cells from individuals with systemic lupus erythematosus express granzyme B. Eur J Immunol 40:2060–2069. https://doi.org/10.1002/eji.200940113. (PMID: 10.1002/eji.20094011320394077)
Lindner S, Dahlke K, Sontheimer K, Hagn M, Kaltenmeier C, Barth TFE, Beyer T, Reister F, Fabricius D, Lotfi R, Lunov O, Nienhaus GU, Simmet T, Kreienberg R, Möller P, Schrezenmeier H, Jahrsdorfer B (2013) Interleukin 21-induced granzyme B-expressing B cells infiltrate tumors and regulate T cells. Cancer res 73:2468. https://doi.org/10.1158/0008-5472.CAN-12-3450. (PMID: 10.1158/0008-5472.CAN-12-345023384943)
Xu W, Narayanan P, Kang N, Clayton S, Ohne Y, Shi P, Herve M-C, Balderas R, Picard C, Casanova J-L, Gorvel J-P, Oh S, Pascual V, Banchereau J (2014) Human plasma cells express granzyme B. Eur J Immunol 44:275–284. https://doi.org/10.1002/eji.201343711. (PMID: 10.1002/eji.20134371124114594)
Hagn M, Blackwell SE, Beyer T, Ebel V, Fabricius D, Lindner S, Stilgenbauer S, Simmet T, Tam C, Neeson P, Trapani JA, Schrezenmeier H, Weiner GJ, Jahrsdörfer B (2014) B-CLL cells acquire APC- and CTL-like phenotypic characteristics after stimulation with CpG ODN and IL-21. Int Immunol 26:383–395. https://doi.org/10.1093/intimm/dxu001. (PMID: 10.1093/intimm/dxu001244976114133571)
Chesneau M, Michel L, Dugast E, Chenouard A, Baron D, Pallier A, Durand J, Braza F, Guerif P, Laplaud D-A, Soulillou J-P, Giral M, Degauque N, Chiffoleau E, Brouard S (2015) Tolerant kidney transplant patients produce B cells with regulatory properties. J Am Soc Nephrol 26:2588. https://doi.org/10.1681/ASN.2014040404. (PMID: 10.1681/ASN.2014040404256441144587683)
Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe JA, Härter G, Grüner B, Fabricius D, Lotfi R, Schwarz K, Schütz C, Hönig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdörfer B (2015) CD4+ T Cell-Derived IL-21 and Deprivation of CD40 Signaling Favor the In Vivo Development of Granzyme B-Expressing Regulatory B Cells in HIV Patients. J Immunol. https://doi.org/10.4049/jimmunol.1402568.
Hagn M, Panikkar A, Smith C, Balfour HH, Khanna R, Voskoboinik I, Trapani JA (2015) B cell-derived circulating granzyme B is a feature of acute infectious mononucleosis. Clin Transl Immunology 4:e38. https://doi.org/10.1038/cti.2015.10. (PMID: 10.1038/cti.2015.10261914094491623)
Zhu J, Zeng Y, Dolff S, Bienholz A, Lindemann M, Brinkhoff A, Schedlowski M, Xu S, Sun M, Guberina H, Kirchhof J, Kribben A, Witzke O, Wilde B (2017) Granzyme B producing B-cells in renal transplant patients. Clin Immunol 184:48–53. https://doi.org/10.1016/j.clim.2017.04.016. (PMID: 10.1016/j.clim.2017.04.01628461110)
Rabani M, Wilde B, Hübbers K, Xu S, Kribben A, Witzke O, Dolff S (2018) IL-21 dependent Granzyme B production of B-cells is decreased in patients with lupus nephritis. Clin Immunol 188:45–51. https://doi.org/10.1016/j.clim.2017.12.005. (PMID: 10.1016/j.clim.2017.12.00529274388)
Arabpour M, Rasolmali R, Talei A-R, Mehdipour F, Ghaderi A (2019) Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes. Mol Immunol 114:172–178. https://doi.org/10.1016/j.molimm.2019.07.019. (PMID: 10.1016/j.molimm.2019.07.01931357083)
Bulati M, Buffa S, Martorana A, Candore G, Lio D, Caruso C, Colonna-Romano G (2014) Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(−)CD27(−)) in the elderly. Exp Gerontol 54:123–129. https://doi.org/10.1016/j.exger.2013.12.011. (PMID: 10.1016/j.exger.2013.12.01124389059)
Hagn M, Sontheimer K, Dahlke K, Brueggemann S, Kaltenmeier C, Beyer T, Hofmann S, Lunov O, Barth TFE, Fabricius D, Tron K, Nienhaus GU, Simmet T, Schrezenmeier H, Jahrsdörfer B (2012) Human B cells differentiate into granzyme B-secreting cytotoxic B lymphocytes upon incomplete T-cell help. Immunol Cell Biol 90:457–467. https://doi.org/10.1038/icb.2011.64. (PMID: 10.1038/icb.2011.6421808264)
Hagn M, Jahrsdörfer B (2012) Why do human B cells secrete granzyme B? Insights into a novel B-cell differentiation pathway. Onco Targets Ther 1:1368–1375. https://doi.org/10.4161/onci.22354. (PMID: 10.4161/onci.22354)
Xu L, Liu X, Liu H, Zhu L, Zhu H, Zhang J, Ren L, Wang P, Hu F, Su Y (2017) Impairment of Granzyme B-producing regulatory B cells correlates with exacerbated rheumatoid arthritis. Front Immunol 8:768. https://doi.org/10.3389/fimmu.2017.00768. (PMID: 10.3389/fimmu.2017.00768287133865491972)
Papp G, bor, Gyimesi E, Szab, Krisztina, Z, Ld, va, Zeher M (2016) Increased IL-21 expression induces granzyme B in peripheral CD5. Mediators of inflammation 2016. https://doi.org/10.1155/2016/4328372.
Contributed Indexing:
Keywords: Breg; Expansion; Granzyme B; Regulation; T/B-cell coculture
Substance Nomenclature:
EC 3.4.21.- (GZMB protein, human)
EC 3.4.21.- (Granzymes)
Entry Date(s):
Date Created: 20210122 Date Completed: 20210402 Latest Revision: 20210402
Update Code:
20240105
DOI:
10.1007/978-1-0716-1237-8_11
PMID:
33479900
Czasopismo naukowe
Granzyme B (GZMB)-expressing B cells inhibit CD4 + T-lymphocyte proliferation in a contact- and GZMB-dependent manner, through degradation of TCR zeta or induction of T-cell apoptosis. This regulatory B-cell population is present in human healthy individuals and represents about 1% of circulating B cells. Their small proportion requires the development of expansion methods to enable their study and envision clinical applications. We describe here how to expand GZMB-expressing B cells to obtain more than 90% of highly purified GZMB + B cells, and the protocol of B/T cells coculture for the evaluation of the suppressive function of the GZMB + B-cell population.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies