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Tytuł pozycji:

Methods to Study the Transcriptome of Regulatory B Cells.

Tytuł :
Methods to Study the Transcriptome of Regulatory B Cells.
Autorzy :
Maho-Vaillant M; INSERM U1234, Normandy University, Rouen, France.; Dermatology Department, Rouen University Hospital, Rouen, France.
Calbo S; INSERM U1234, Normandy University, Rouen, France. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2270, pp. 295-305.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms :
B-Lymphocytes, Regulatory/*metabolism
Gene Expression Profiling/*methods
Transcriptome/*genetics
Animals ; B-Lymphocyte Subsets/cytology ; B-Lymphocyte Subsets/metabolism ; B-Lymphocytes/cytology ; B-Lymphocytes/metabolism ; B-Lymphocytes, Regulatory/cytology ; Cell Lineage/genetics ; Humans ; Mice ; Oligonucleotide Array Sequence Analysis/methods ; Real-Time Polymerase Chain Reaction/methods
References :
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Rincon-Arevalo H, Sanchez-Parra CC, Castano D, Yassin L, Vasquez G (2016) Regulatory B cells and mechanisms. Int Rev Immunol 35(2):156–176. https://doi.org/10.3109/08830185.2015.1015719. (PMID: 10.3109/08830185.2015.101571925793964)
Rosser EC, Mauri C (2015) Regulatory B cells: origin, phenotype, and function. Immunity 42(4):607–612. https://doi.org/10.1016/j.immuni.2015.04.005. (PMID: 10.1016/j.immuni.2015.04.00525902480)
Zheng GX, Terry JM, Belgrader P, Ryvkin P, Bent ZW, Wilson R, Ziraldo SB, Wheeler TD, McDermott GP, Zhu J, Gregory MT, Shuga J, Montesclaros L, Underwood JG, Masquelier DA, Nishimura SY, Schnall-Levin M, Wyatt PW, Hindson CM, Bharadwaj R, Wong A, Ness KD, Beppu LW, Deeg HJ, McFarland C, Loeb KR, Valente WJ, Ericson NG, Stevens EA, Radich JP, Mikkelsen TS, Hindson BJ, Bielas JH (2017) Massively parallel digital transcriptional profiling of single cells. Nat Commun 8:14049. https://doi.org/10.1038/ncomms14049. (PMID: 10.1038/ncomms14049280916015241818)
Shalek AK, Satija R, Adiconis X, Gertner RS, Gaublomme JT, Raychowdhury R, Schwartz S, Yosef N, Malboeuf C, Lu D, Trombetta JJ, Gennert D, Gnirke A, Goren A, Hacohen N, Levin JZ, Park H, Regev A (2013) Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells. Nature 498(7453):236–240. https://doi.org/10.1038/nature12172. (PMID: 10.1038/nature12172236854543683364)
Berkani N, Joly P, Golinski ML, Colliou N, Lim A, Larbi A, Riou G, Caillot F, Bernard P, Bedane C, Delaporte E, Chaby G, Dompmartin A, Hertl M, Calbo S, Musette P (2019) B-cell depletion induces a shift in self antigen specific B-cell repertoire and cytokine pattern in patients with bullous pemphigoid. Sci Rep 9(1):3525. https://doi.org/10.1038/s41598-019-40203-7. (PMID: 10.1038/s41598-019-40203-7308376356401188)
Hebert V, Petit M, Maho-Vaillant M, Golinski ML, Riou G, Derambure C, Boyer O, Joly P, Calbo S (2019) Modifications of the Transcriptomic profile of autoreactive B cells from pemphigus patients after treatment with rituximab or a standard corticosteroid regimen. Front Immunol 10:1794. https://doi.org/10.3389/fimmu.2019.01794. (PMID: 10.3389/fimmu.2019.01794314402356693356)
Golinski ML, Demeules M, Derambure C, Riou G, Maho-Vaillant M, Boyer O, Joly P, Calbo S (2020) CD11c(+) B cells are mainly memory cells, precursors of antibody secreting cells in healthy donors. Front Immunol 11:32. https://doi.org/10.3389/fimmu.2020.00032. (PMID: 10.3389/fimmu.2020.00032321584427051942)
Lin W, Cerny D, Chua E, Duan K, Yi JT, Shadan NB, Lum J, Maho-Vaillant M, Zolezzi F, Wong SC, Larbi A, Fink K, Musette P, Poidinger M, Calbo S (2014) Human regulatory B cells combine phenotypic and genetic hallmarks with a distinct differentiation fate. J Immunol 193(5):2258–2266. https://doi.org/10.4049/jimmunol.1303214. (PMID: 10.4049/jimmunol.130321425080484)
Contributed Indexing :
Keywords: B Cells*; Gene expression profiling*; Microarrays*; RT-qPCR*; mRNA*
Entry Date(s) :
Date Created: 20210122 Date Completed: 20210402 Latest Revision: 20210402
Update Code :
20210403
DOI :
10.1007/978-1-0716-1237-8_16
PMID :
33479905
Czasopismo naukowe
Regulatory B cells do not constitute a distinct cell lineage because no unique marker or set of markers can exclusively identify neither murine nor human regulatory B cells, and efficient IL-10 production is their only known distinguishing feature. After purification of IL-10-secreting B cells, one may want to characterize them by analyzing their gene expression profile. This goal can be achieved by using different technologies: RT-qPCR, microarrays, Nanostring's nCounter technology, Biomark HD are techniques that will allow you to analyze their gene expression, whether in a targeted (RT-qPCR), extended but targeted (Nanostring's nCounter technology, Biomark HD) or exhaustive (Microarray) way. Aim of this chapter is the description of these techniques in the view of their application to the study and characterization of regulatory B cells.

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