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Tytuł pozycji:

Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.

Tytuł:
Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.
Autorzy:
El-Dein AN; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Div., National Research Centre, Giza, Egypt.
Nour El-Deen AM; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Div., National Research Centre, Giza, Egypt.
El-Shatoury EH; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Awad GA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Div., National Research Centre, Giza, Egypt.
Ibrahim MK; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Awad HM; Tanning Materials and Leather Technology Department, National Research Centre, Giza, Egypt.
Farid MA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Div., National Research Centre, Giza, Egypt. Electronic address: .
Źródło:
International journal of biological macromolecules [Int J Biol Macromol] 2021 Mar 15; Vol. 173, pp. 66-78. Date of Electronic Publication: 2021 Jan 19.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
MeSH Terms:
Anticholesteremic Agents/*pharmacology
Antineoplastic Agents/*pharmacology
Antioxidants/*pharmacology
Hypercholesterolemia/*therapy
Polysaccharides, Bacterial/*pharmacology
Probiotics/*pharmacology
Animals ; Anticholesteremic Agents/isolation & purification ; Antineoplastic Agents/isolation & purification ; Antioxidants/isolation & purification ; Bacteriocins ; Cell Survival/drug effects ; Cholesterol, HDL/agonists ; Cholesterol, HDL/metabolism ; Cholesterol, LDL/antagonists & inhibitors ; Cholesterol, LDL/metabolism ; Disease Models, Animal ; Egypt ; Fermented Foods/microbiology ; HCT116 Cells ; Hep G2 Cells ; Humans ; Hypercholesterolemia/metabolism ; Hypercholesterolemia/pathology ; Lactobacillus plantarum/chemistry ; Lactobacillus plantarum/metabolism ; Lacticaseibacillus rhamnosus/chemistry ; Lacticaseibacillus rhamnosus/metabolism ; Male ; PC-3 Cells ; Polysaccharides, Bacterial/isolation & purification ; Probiotics/isolation & purification ; Rats ; Rats, Wistar ; Triglycerides/antagonists & inhibitors ; Triglycerides/metabolism
Contributed Indexing:
Keywords: Bacteriocins; Exopolysaccharides; Hypocholesterolemic potential; In vivo application; Probiotics
Substance Nomenclature:
0 (Anticholesteremic Agents)
0 (Antineoplastic Agents)
0 (Antioxidants)
0 (Bacteriocins)
0 (Cholesterol, HDL)
0 (Cholesterol, LDL)
0 (Polysaccharides, Bacterial)
0 (Triglycerides)
Entry Date(s):
Date Created: 20210122 Date Completed: 20210430 Latest Revision: 20221207
Update Code:
20240105
DOI:
10.1016/j.ijbiomac.2021.01.107
PMID:
33482208
Czasopismo naukowe
Lactobacilli probiotics have been suggested to reduce cholesterol with low side effects to host. Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry. Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties. Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar. The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy. Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively. Their EPSs showed good anticancer activities against carcinoma cells with low IC 50 values for HCT-116, PC-3 and HepG-2 cells. To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3. The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities. In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains. Whereas, HDL was increased by about 43 and 38%, respectively, and the atherogenic indices decreased.
Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest.
(Copyright © 2021. Published by Elsevier B.V.)

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