-
Tytuł:
-
Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles.
-
Autorzy:
-
Li Z; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.; College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.
Ali I; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.; Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan.
Qiu J; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
Zhao H; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.
Ma W; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.; College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.
Bai A; Jinan Municipal Center for Disease Control and Prevention, Jinan 250001, People's Republic of China.
Wang D; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.
Li J; Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.
-
Źródło:
-
International journal of nanomedicine [Int J Nanomedicine] 2021 Jan 19; Vol. 16, pp. 481-492. Date of Electronic Publication: 2021 Jan 19 (Print Publication: 2021).
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Auckland : DOVE Medical Press,
-
MeSH Terms:
-
Anti-Bacterial Agents/*pharmacology
Antineoplastic Agents/*pharmacology
Antioxidants/*pharmacology
Flavonols/*pharmacology
Metal Nanoparticles/*chemistry
Silver/*pharmacology
Bacteria/drug effects ; Cell Line, Tumor ; Escherichia coli/drug effects ; Humans ; Metal Nanoparticles/ultrastructure ; Microbial Sensitivity Tests ; Spectrophotometry, Ultraviolet ; X-Ray Diffraction
-
References:
-
Oncol Lett. 2020 Jul;20(1):357-363. (PMID: 32565961)
Food Chem. 2015 Apr 1;172:416-22. (PMID: 25442572)
Gen Pharmacol. 1997 Aug;29(2):121-6. (PMID: 9251891)
Appl Microbiol Biotechnol. 2016 Jan;100(2):521-34. (PMID: 26300292)
Microbes Infect. 2020 Jul - Aug;22(6-7):254-262. (PMID: 32554102)
Life Sci. 2019 Apr 15;223:62-68. (PMID: 30872178)
J Food Biochem. 2020 Mar;44(3):e13143. (PMID: 31910490)
Int J Mol Sci. 2018 Nov 16;19(11):. (PMID: 30453526)
Molecules. 2019 Aug 03;24(15):. (PMID: 31382605)
Int J Nanomedicine. 2020 Aug 24;15:6295-6310. (PMID: 32943863)
Food Chem. 2017 Mar 1;218:463-470. (PMID: 27719936)
Int J Nanomedicine. 2020 Sep 21;15:6905-6916. (PMID: 33061358)
J Cell Mol Med. 2020 May;24(10):5911-5925. (PMID: 32301289)
Molecules. 2018 Aug 30;23(9):. (PMID: 30200189)
Biochem Pharmacol. 2020 May;175:113927. (PMID: 32217100)
Int J Nanomedicine. 2018 Dec 12;13:8537-8548. (PMID: 30587976)
J Nanosci Nanotechnol. 2020 Oct 1;20(10):6148-6154. (PMID: 32384964)
J Adv Res. 2017 Nov;8(6):561-568. (PMID: 28765792)
IET Nanobiotechnol. 2017 Dec;11(8):973-980. (PMID: 29155397)
Biosci Biotechnol Biochem. 2018 Jan;82(1):135-138. (PMID: 29235425)
Mol Plant. 2012 Mar;5(2):472-81. (PMID: 22115918)
Front Pharmacol. 2018 Oct 25;9:1204. (PMID: 30410442)
J Photochem Photobiol B. 2016 Aug;161:200-10. (PMID: 27261701)
Oncotarget. 2015 Feb 20;6(5):3254-67. (PMID: 25226612)
Int J Nanomedicine. 2015 Mar 31;10:2567-77. (PMID: 25848272)
Int J Nanomedicine. 2020 May 28;15:3791-3801. (PMID: 32547028)
Sci Rep. 2017 Jun 6;7(1):2823. (PMID: 28588273)
Cardiovasc Drugs Ther. 2020 Jun;34(3):291-301. (PMID: 32212062)
J Mol Model. 2018 May 11;24(6):133. (PMID: 29752542)
Anticancer Drugs. 2017 Mar;28(3):281-288. (PMID: 27997436)
Molecules. 2017 Apr 21;22(4):. (PMID: 28430149)
J Adv Res. 2016 Jan;7(1):17-28. (PMID: 26843966)
Artif Cells Nanomed Biotechnol. 2018 Sep;46(6):1163-1170. (PMID: 28784039)
J Photochem Photobiol B. 2018 Jan;178:463-471. (PMID: 29223813)
-
Contributed Indexing:
-
Keywords: DMY-AgNO3-NPs; DPPH; Escherichia coli; Hela; HepG2; MDA-MB-231; Salmonella; green approach; natural flavonoid
-
Substance Nomenclature:
-
0 (Anti-Bacterial Agents)
0 (Antineoplastic Agents)
0 (Antioxidants)
0 (Flavonols)
3M4G523W1G (Silver)
KD8QND6427 (dihydromyricetin)
-
Entry Date(s):
-
Date Created: 20210127 Date Completed: 20210211 Latest Revision: 20220420
-
Update Code:
-
20240105
-
PubMed Central ID:
-
PMC7826068
-
DOI:
-
10.2147/IJN.S283677
-
PMID:
-
33500618
-
Background: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli ( E. coli), and Salmonella . In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.
Methods: The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.
Results: The results showed that the dispersion of AgNPs had the maximum UV-visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01-0.1mg/mL), the free radical scavenging rate was about 56-92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli ( E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10 -6 g/L, and 10 -4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.
Conclusion: The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.
Competing Interests: The authors report no conflicts of interest in this work.
(© 2021 Li et al.)
