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Tytuł:
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TRITC-Loaded PLGA Nanoparticles as Drug Delivery Carriers in Mouse Oocytes and Embryos.
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Autorzy:
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Kim HJ; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Lee S; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Lee JH; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Park JM; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Hong SJ; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Lee OH; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Park JS; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
Choi Y; Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
Park KH; Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si 13488, Republic of Korea.
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Źródło:
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ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Feb 10; Vol. 13 (5), pp. 5975-5988. Date of Electronic Publication: 2021 Jan 27.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, D.C. : American Chemical Society
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MeSH Terms:
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Drug Delivery Systems*
Nanoparticles/*chemistry
Oocytes/*drug effects
Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry
Rhodamines/*chemistry
Animals ; Cell Survival/drug effects ; Drug Carriers/chemistry ; Female ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Molecular Structure ; Particle Size ; Polyethylene Glycols/chemistry ; Rhodamines/pharmacology ; Surface Properties
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Contributed Indexing:
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Keywords: genetic stability; mouse oocyte; oocyte maturation; poly(lactic-co-glycolic acid) (PLGA); preimplantation embryonic development; tetramethylrhodamine (TRITC)
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Substance Nomenclature:
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0 (Drug Carriers)
0 (Rhodamines)
1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
3WJQ0SDW1A (Polyethylene Glycols)
62669-72-1 (tetramethylrhodamine)
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Entry Date(s):
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Date Created: 20210127 Date Completed: 20210304 Latest Revision: 20210304
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Update Code:
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20240105
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DOI:
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10.1021/acsami.0c19792
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PMID:
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33502166
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The structural layers around oocytes make it difficult to deliver drugs aimed at treating infertility. In this study, we sought to identify nanoparticles (NPs) that could easily pass through zona pellucida (ZP), a special layer around oocytes, for use as a drug delivery carrier. Three types of NPs were tested: quantum dot NPs, PE-polyethylene glycol (PEG)-loaded poly(lactic- co -glycolic acid) (PLGA) NPs (PEG/PL), and tetramethylrhodamine-loaded PLGA NPs (TRNPs). When mouse oocytes were treated with NPs, only TRNPs could fully pass through the ZP and cell membrane. To assess the effects of TRNPs on fertility and potential nanotoxicity, we performed mRNA sequencing analysis to confirm their genetic safety. We established a system to successfully internalize TRNPs into oocytes. The genetic stability and normal development of TRNP-treated oocytes and embryos were confirmed. These results imply that TRNPs can be used as a drug delivery carrier applicable to germ cells.
