Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon.

Szczegóły
Abstrakt

Tytuł:: Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon.
Autorzy:: Jan Z; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Drab M; Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia.
Drobne D; Nanobiology and Nanotoxicology Group, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Bedina Zavec A; Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia.
Benčina M; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Ljubljana, Slovenia.
Drasler B; Nanobiology and Nanotoxicology Group, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Hočevar M; Physics and Chemistry of Materials, Laboratory of Surface Engineering and Applied Surface Science, The Institute of Metals and Technology, Ljubljana, Slovenia.
Krek JL; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Pađen L; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Pajnič M; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Repar N; Nanobiology and Nanotoxicology Group, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Šimunič B; Institute for Kinesiology Research, Science and Research Centre Koper, Koper, Slovenia.
Štukelj R; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Kralj-Iglič V; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
Źródło:: International journal of nanomedicine [Int J Nanomedicine] 2021 Jan 20; Vol. 16, pp. 443-456. Date of Electronic Publication: 2021 Jan 20 (Print Publication: 2021).
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Auckland : DOVE Medical Press,
MeSH Terms:: Athletes*
Marathon Running*
Blood/*metabolism
Nanoparticles/*chemistry
Adult ; Blood Cell Count ; C-Reactive Protein/analysis ; Erythrocytes/metabolism ; Female ; Flow Cytometry ; Humans ; Interleukin-6/blood ; Lipids/chemistry ; Male ; Middle Aged ; Physical Exertion/physiology ; Tumor Necrosis Factor-alpha/blood ; Young Adult
References:: Epigenet Insights. 2018 Dec 12;11:2516865718818839. (PMID: 30560231)
Biomed Res Int. 2020 Apr 18;2020:6381396. (PMID: 32382562)
Cells. 2019 Sep 06;8(9):. (PMID: 31500151)
Int J Environ Health Res. 2012;22(3):279-86. (PMID: 22149082)
J Appl Physiol (1985). 2005 Apr;98(4):1154-62. (PMID: 15772055)
Med Sci Sports Exerc. 2011 Jul;43(7):1169-76. (PMID: 21131870)
Br J Haematol. 2015 Dec;171(5):854-61. (PMID: 26456230)
J Gerontol A Biol Sci Med Sci. 2006 Jun;61(6):575-84. (PMID: 16799139)
Biochem Genet. 2016 Aug;54(4):544-551. (PMID: 27368697)
J Extracell Vesicles. 2015 Jul 02;4:28239. (PMID: 26142461)
Am J Physiol Heart Circ Physiol. 2016 Nov 1;311(5):H1297-H1310. (PMID: 27638881)
Clin Hemorheol Microcirc. 2011;47(1):15-25. (PMID: 21321404)
Stem Cells. 2020 Feb;38(2):291-300. (PMID: 31675158)
Lipids Health Dis. 2011 Mar 21;10:47. (PMID: 21418650)
Cell Metab. 2018 Jan 9;27(1):237-251.e4. (PMID: 29320704)
J Pharm Bioallied Sci. 2011 Jan;3(1):118-27. (PMID: 21430962)
J Leukoc Biol. 2008 Nov;84(5):1271-8. (PMID: 18664531)
Small. 2018 Sep;14(39):e1801650. (PMID: 30160371)
Mediators Inflamm. 2019 Apr 17;2019:9213074. (PMID: 31148950)
Electrophoresis. 2020 Apr;41(7-8):449-470. (PMID: 31967658)
Cardiovasc Diabetol. 2017 Sep 19;16(1):118. (PMID: 28927403)
J Biol Chem. 1987 Jul 5;262(19):9412-20. (PMID: 3597417)
J Extracell Vesicles. 2019 Jul 03;8(1):1628592. (PMID: 31303981)
Arch Dis Child Fetal Neonatal Ed. 1999 Sep;81(2):F130-3. (PMID: 10448182)
Cytometry A. 2016 Feb;89(2):123-34. (PMID: 26651033)
J Physiol. 2004 Jul 15;558(Pt 2):633-45. (PMID: 15169848)
J Pathol. 2008 Jan;214(2):149-60. (PMID: 18161752)
Eur J Appl Physiol. 2011 Jun;111(6):1035-45. (PMID: 21088973)
Int J Nanomedicine. 2011;6:2737-48. (PMID: 22128248)
Virol J. 2010 Sep 16;7:234. (PMID: 20846372)
Cell. 2010 Dec 10;143(6):875-87. (PMID: 21145455)
J Extracell Vesicles. 2019 May 28;8(1):1615820. (PMID: 31191831)
Life Sci. 2020 Jun 1;250:117585. (PMID: 32243928)
Oncotarget. 2018 Mar 30;9(24):17181-17198. (PMID: 29682215)
J Immunol Methods. 2008 Sep 30;338(1-2):21-30. (PMID: 18675270)
Eur J Appl Physiol Occup Physiol. 1992;64(4):354-8. (PMID: 1592062)
Biochem Pharmacol. 1961 Jul;7:88-95. (PMID: 13726518)
Antioxidants (Basel). 2017 Sep 28;6(4):. (PMID: 28956814)
Biomed Res Int. 2018 Mar 26;2018:5670145. (PMID: 29780825)
Crit Rev Immunol. 2002;22(3):165-82. (PMID: 12498381)
J Appl Physiol (1985). 2017 Apr 1;122(4):1031-1039. (PMID: 28153942)
J Diabetes Res. 2018 Jun 19;2018:7807245. (PMID: 30018986)
Adv Enzymol Relat Areas Mol Biol. 1985;57:357-417. (PMID: 3898742)
Free Radic Biol Med. 2020 Mar;149:44-50. (PMID: 31783096)
Circ Res. 2014 Jan 3;114(1):109-13. (PMID: 24141170)
J Med Biochem. 2015 Apr;34(2):179-190. (PMID: 28356830)
Eur J Biochem. 2000 Jan;267(2):583-90. (PMID: 10632729)
J Extracell Vesicles. 2019 Aug 1;8(1):1648167. (PMID: 31489144)
Nutrients. 2019 Mar 08;11(3):. (PMID: 30857250)
Eur J Pharm Sci. 2017 Feb 15;98:17-29. (PMID: 27737793)
Rheumatology (Oxford). 2017 Mar 1;56(3):408-416. (PMID: 28031442)
Contributed Indexing:: Keywords: blood samples; cellular nanovesicles; inflammation process; marathon; membrane vesiculation; physical effort
Substance Nomenclature:: 0 (Interleukin-6)
0 (Lipids)
0 (Tumor Necrosis Factor-alpha)
9007-41-4 (C-Reactive Protein)
Entry Date(s):: Date Created: 20210128 Date Completed: 20210211 Latest Revision: 20220420
Update Code:: 20240105
PubMed Central ID:: PMC7829122
DOI:: 10.2147/IJN.S282200
PMID:: 33505159
: Czasopismo naukowe

Full Text Finder

Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort.
Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement.
Results: More than 15 hours after physical effort a decrease was found in CNVs' concentration in isolates from blood (46%; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs' concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found.
Conclusion: We found that >15 hours post-physical effort, CNVs' concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs' concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Competing Interests: No conflicts of interest, financial or otherwise, are declared by the authors.
(© 2021 Jan et al.)

Informacja