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Tytuł:
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Optimization of short-term storage of pikeperch semen: an applicable approach.
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Autorzy:
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Kristan J; Faculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech Republic.
Dadras H; Faculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech Republic. Electronic address: .
Policar T; Faculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech Republic.
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Źródło:
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Animal : an international journal of animal bioscience [Animal] 2021 Jan; Vol. 15 (1), pp. 100001. Date of Electronic Publication: 2020 Dec 10.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2021- : [London] : Elsevier
Original Publication: Cambridge, England : Cambridge University Press
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MeSH Terms:
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Semen*
Semen Preservation*/veterinary
Animals ; Female ; Male ; Semen Analysis/veterinary ; Sperm Motility ; Spermatozoa
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Contributed Indexing:
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Keywords: Fish; Immobilizing media; Sander lucioperca; Sperm motility
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Entry Date(s):
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Date Created: 20210130 Date Completed: 20210324 Latest Revision: 20210324
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Update Code:
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20240104
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DOI:
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10.1016/j.animal.2020.100001
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PMID:
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33515997
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Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4°C using two immobilizing media (IM): (a) IM1, 180mM NaCl, 2.68mM KCl, 1.36mM CaCl 2 ⋅2H 2 O and 2.38mM NaHCO 3 , 343mOsm/kg; and (b) IM2, 200mM NaCl, 2.68mM KCl, 1.36mM CaCl 2 ⋅2H 2 O and 2.38mM NaHCO 3 , 381mOsm/kg. Undiluted sperm was used as the control. At 6h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30s postactivation in all groups. Over 48h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment for 2days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries.
(Copyright © 2020. Published by Elsevier B.V.)
