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Tytuł:
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Diagnostic accuracy of Xpert MTB/RIF Ultra for tuberculous meningitis in a clinical practice setting of China.
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Autorzy:
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Huang M; Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Wang G; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Sun Q; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Jiang G; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Li W; Department of Emergency, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Ding Z; Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Jia H; Laboratory of Molecular Biology, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Gao M; Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China.
Huang H; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China. Electronic address: .
Li Q; Clinical Center on Tuberculosis Control, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China. Electronic address: .
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Źródło:
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Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2021 May; Vol. 100 (1), pp. 115306. Date of Electronic Publication: 2021 Jan 06.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: [New York, NY] : Elsevier Biomedical, [c1983-
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MeSH Terms:
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Mycobacterium tuberculosis*/drug effects
Mycobacterium tuberculosis*/genetics
Drug Resistance, Bacterial/*genetics
Molecular Diagnostic Techniques/*methods
Tuberculosis, Meningeal/*diagnosis
Adult ; Antibiotics, Antitubercular/pharmacology ; China ; DNA, Bacterial/cerebrospinal fluid ; Female ; Humans ; Male ; Middle Aged ; Nucleic Acid Amplification Techniques ; Rifampin/pharmacology ; Sensitivity and Specificity ; Tuberculosis, Meningeal/microbiology ; Young Adult
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Contributed Indexing:
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Keywords: Cerebrospinal fluid; Culture; Diagnosis; Tuberculous meningitis; Xpert MTB/RIF Ultra
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Substance Nomenclature:
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0 (Antibiotics, Antitubercular)
0 (DNA, Bacterial)
VJT6J7R4TR (Rifampin)
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Entry Date(s):
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Date Created: 20210131 Date Completed: 20210913 Latest Revision: 20210913
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Update Code:
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20240104
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DOI:
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10.1016/j.diagmicrobio.2020.115306
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PMID:
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33516988
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A comparative performance evaluation of the novel Xpert MTB/RIF Ultra (Xpert Ultra) and MTB/RIF Xpert (Xpert) for tuberculous meningitis (TBM) diagnosis was performed. The cerebrospinal fluids of suspected TBM patients were collected consecutively and subjected to smear microscopy, culture, Xpert, and Xpert Ultra. In total, 160 patients were recruited. Xpert Ultra produced a higher sensitivity (45%, 34 of 76) than Xpert (28%, 21 of 76; P = 0.001) and culture (18%, 14 of 76; P < 0.001), respectively. Inclusion of Xpert Ultra outcomes increased the percentage of definite TBM case from 36% (27 of 76) to 51% (39 of 76). Both Xpert Ultra and Xpert accurately identified the one rifampicin (RIF)-resistant and the 5 RIF-sensitive cases defined by phenotypic drug sensitivity test. The specificities of all of the culture, Xpert and Xpert Ultra were 100% (45 of 45). Xpert Ultra outperformed both Xpert and culture for TBM diagnosis, which may speed up the appropriate treatment of patients in clinical practice.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
