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Tytuł:
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Lapathoside A Isolated from Fagopyrum esculentum Induces Apoptosis in Human Pancreatic Cancer Cells.
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Autorzy:
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Kang MS; Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju, Republic of Korea.
Ham YM; Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
Oh DJ; Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
Jung YH; Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
Han SI; Subtropical/Tropical organism gene bank, Jeju National University, Jeju, Republic of Korea .
Kim JH; Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju, Republic of Korea; .; Subtropical/Tropical organism gene bank, Jeju National University, Jeju, Republic of Korea.
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Źródło:
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Anticancer research [Anticancer Res] 2021 Feb; Vol. 41 (2), pp. 747-756.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Attiki, Greece : International Institute of Anticancer Research
Original Publication: Athens, Greece : Potamitis Press
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MeSH Terms:
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Fagopyrum*/chemistry
Antineoplastic Agents, Phytogenic/*pharmacology
Apoptosis/*drug effects
Cinnamates/*pharmacology
Pancreatic Neoplasms/*drug therapy
Antineoplastic Agents, Phytogenic/isolation & purification ; Apoptosis Regulatory Proteins/metabolism ; Cell Line, Tumor ; Cinnamates/isolation & purification ; Humans ; Pancreatic Neoplasms/pathology ; Signal Transduction
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Contributed Indexing:
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Keywords: Buckwheat root; anticancer activity; lapathoside A; pancreatic cancer
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Substance Nomenclature:
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0 (Antineoplastic Agents, Phytogenic)
0 (Apoptosis Regulatory Proteins)
0 (Cinnamates)
0 (lapathoside A)
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Entry Date(s):
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Date Created: 20210131 Date Completed: 20210208 Latest Revision: 20210208
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Update Code:
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20240104
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DOI:
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10.21873/anticanres.14826
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PMID:
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33517279
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Background/aim: Lapathoside A, a phenylpropanoid ester, was isolated from the roots of buckwheat by searching for bioactive compounds against human pancreatic cancer cells.
Materials and Methods: Buckwheat root extracts, prepared by 70% ethanol, were separated into n-hexane, methylene chloride, ethyl acetate, n-butanol, and water fraction by solvent partitioning. Seven fractions were obtained from the ethyl acetate fraction by liquid chromatography, and fraction No. 6 contained lapathoside A. The effects of lapathoside A on Panc-1 and SNU-213 human pancreatic cancer cell lines were examined.
Results: The structure of lapathoside A was determined by liquid chromatography-mass spectrometry, liquid chromatography-tandem mass spectrometry, and nuclear magnetic resonance analysis. Next, we investigated whether lapathoside A has anticancer activity in human pancreatic cancer cell lines (PANC-1 and SNU-213). After treatment with 25 μM lapathoside A, viability of PANC-1 and SNU-213 cells decreased to about 40 and 27%, respectively. In addition, lapathoside A treatment also increased apoptosis while affecting the expression levels of apoptotic proteins.
Conclusion: The effect of lapathoside A on apoptosis was confirmed in pancreatic cancer cell lines, supporting the application of lapathoside A in the treatment of pancreatic cancer.
(Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
