Cell wall thickness and the molecular mechanism of heterogeneous vancomycin-intermediate Staphylococcus aureus.

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Tytuł:: Cell wall thickness and the molecular mechanism of heterogeneous vancomycin-intermediate Staphylococcus aureus.
Autorzy:: Cui J; Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.
Zhang H; Department of Respiratory disease, Beijing Luhe Hospital Affiliated to Capital Medical University, Beijing, China.
Mo Z; Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.
Yu M; Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.
Liang Z; Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.
Źródło:: Letters in applied microbiology [Lett Appl Microbiol] 2021 May; Vol. 72 (5), pp. 604-609. Date of Electronic Publication: 2021 Feb 15.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2023- : Oxford : Oxford University Press
Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985-
MeSH Terms:: Cell Wall/*metabolism
Cell Wall/*physiology
Methicillin-Resistant Staphylococcus aureus/*drug effects
Vancomycin Resistance/*genetics
Vancomycin-Resistant Staphylococcus aureus/*growth & development
Anti-Bacterial Agents/pharmacology ; Gene Expression Regulation, Bacterial/genetics ; Humans ; Methicillin/pharmacology ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/growth & development ; Microbial Sensitivity Tests ; Staphylococcal Infections/drug therapy ; Up-Regulation/genetics ; Vancomycin/pharmacology
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Grant Information:: 16CXZ041 Chinese Military Medical Innovation Project
Contributed Indexing:: Keywords: MRSA; RT-PCR; TEM; cell wall thickness; hVISA; molecular mechanism
Substance Nomenclature:: 0 (Anti-Bacterial Agents)
6Q205EH1VU (Vancomycin)
Q91FH1328A (Methicillin)
Entry Date(s):: Date Created: 20210204 Date Completed: 20210427 Latest Revision: 20210703
Update Code:: 20240105
PubMed Central ID:: PMC8248079
DOI:: 10.1111/lam.13456
PMID:: 33539564
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Methicillin-resistant Staphylococcus aureus (MRSA) with reduced sensitivity to vancomycin (VAN) has caused many clinical cases of VAN treatment failure, but the molecular mechanism underlying the reduced sensitivity to VAN is still unclear. We isolated a heterogeneous VAN-intermediate Staphylococcus aureus (hVISA), which was also a MRSA strain with reduced sensitivity to VAN. To investigate the molecular mechanism underlying the reduced sensitivity to VAN exhibited by the hVISA strain, we compared the hVISA strain with a VAN-sensitive MRSA strain, known as the N315 strain. The images captured by transmission electron microscopy showed that the cell wall of the hVISA strain was significantly thicker than that of the N315 strain (36·72 ± 1·04 nm vs 28·15 ± 1·25 nm, P < 0·05), and the results of real-time quantitative PCR analysis suggested that the expression levels of the cell wall thickness related genes (glmS, vraR/S, sgtB, murZ and PBP4) of the hVISA strain were significantly higher than those of the N315 strain (P < 0·05). In conclusion, this study indicated that the upregulation of the expression of the genes related to cell wall synthesis might be the molecular mechanism underlying the cell wall thickening of the hVISA strain and might be related to its resistance to VAN.
(© 2021 The Authors. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.)

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