Microparticles: a link to increased thrombin generation.

Szczegóły
Abstrakt

Tytuł:: Microparticles: a link to increased thrombin generation.
Autorzy:: Gasa N; Department of Haematology and Cell Biology, Faculty of Health Sciences, University of the Free State.; Department of Haematology and Cell Biology, Universitas Academic Business Unit, National Health Laboratory Service, Bloemfontein, South Africa.
Meiring M; Department of Haematology and Cell Biology, Faculty of Health Sciences, University of the Free State.; Department of Haematology and Cell Biology, Universitas Academic Business Unit, National Health Laboratory Service, Bloemfontein, South Africa.
Źródło:: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis [Blood Coagul Fibrinolysis] 2021 Apr 01; Vol. 32 (3), pp. 204-208.
Typ publikacji:: Journal Article; Observational Study
Język:: English
Imprint Name(s):: Publication: London : Lippincott Williams And Wilkins
Original Publication: Oxford, UK : Rapid Communications of Oxford Ltd., c1990-
MeSH Terms:: Blood Coagulation*
Cell-Derived Microparticles/*metabolism
Thrombin/*metabolism
Thrombophilia/*metabolism
Blood Coagulation Tests ; Humans ; Thrombin/analysis ; Thrombophilia/blood
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Substance Nomenclature:: EC 3.4.21.5 (Thrombin)
Entry Date(s):: Date Created: 20210209 Date Completed: 20210623 Latest Revision: 20210623
Update Code:: 20240104
DOI:: 10.1097/MBC.0000000000001018
PMID:: 33560006
: Czasopismo naukowe

Circulating microparticles in human plasma may play a significant role in thrombogenesis because they carry the initiator of blood coagulation, tissue factor. Microparticles in blood are derived from diverse cell types, including erythrocytes, endothelial cells and platelets. Thrombin generation is an important part of the coagulation system and might be influenced by the presence of microparticles in the circulation. With this study, we determined the contribution of microparticles to increased thrombin generation in plasma samples received for thrombophilia workup and compare that with normal plasma. Microparticles were isolated from 50 plasma samples with increased thrombin generation and 20 plasma samples with normal thrombin generation, using filtration. Thrombin generation assay were performed by adding a low concentration of tissue factor-containing phospholipids and a fluorescence substrate for thrombin formation to plasma samples and measuring fluorescence at 1-min intervals over a period of 90 min on all samples (with and without the presence of microparticles). The peak thrombin, velocity-index and area under the curve were calculated. Microparticles contribute to the different parameters in samples with increased thrombin generation as follows: 50 ± 19% for peak thrombin, 58 ± 24% for velocity-index and 35 ± 13% for area under the curve. Microparticles did not contribute to thrombin generation in plasma samples with normal thrombin generation. Microparticles play a significant role in coagulation and contribute largely to increased thrombin generation in plasma; however, microparticles do not contribute to coagulation in the plasma of participants with normal thrombin generation.
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