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Tytuł:
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Stress-responsive tomato gene SlGRAS4 function in drought stress and abscisic acid signaling.
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Autorzy:
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Liu Y; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Wen L; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Shi Y; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Su D; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Lu W; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Cheng Y; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China.
Li Z; Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China; Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China. Electronic address: .
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Źródło:
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Plant science : an international journal of experimental plant biology [Plant Sci] 2021 Mar; Vol. 304, pp. 110804. Date of Electronic Publication: 2020 Dec 25.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: <2010>- : Shannon, Co. Clare : Elsevier Ireland
Original Publication: Shannon [Clare] : Elsevier Scientific Publishers Ireland Ltd., c1985-
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MeSH Terms:
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Signal Transduction*/physiology
Abscisic Acid/*metabolism
Genes, Plant/*physiology
Solanum lycopersicum/*genetics
Plant Growth Regulators/*metabolism
Plant Proteins/*physiology
Dehydration ; Germination ; Solanum lycopersicum/metabolism ; Solanum lycopersicum/physiology ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Reactive Oxygen Species/metabolism ; Seedlings/growth & development ; Transcriptome ; Two-Hybrid System Techniques
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Contributed Indexing:
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Keywords: ABA signaling; Abscisic acid; Drought stress; GRAS; ROS; Tomato (Solanumlycopersicum)
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Substance Nomenclature:
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0 (Plant Growth Regulators)
0 (Plant Proteins)
0 (Reactive Oxygen Species)
72S9A8J5GW (Abscisic Acid)
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Entry Date(s):
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Date Created: 20210211 Date Completed: 20210517 Latest Revision: 20221207
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Update Code:
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20240105
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DOI:
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10.1016/j.plantsci.2020.110804
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PMID:
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33568303
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Adverse environmental conditions such as drought stress greatly limit the growth and production of crops worldwide. In this study, SlGRAS4, a drought stress-responsive GRAS gene from tomato (Solanum lycopersicum) was functionally characterized. Repressing SlGRAS4 (SlGRAS4-RNAi) increased sensitivity to drought stress, whereas overexpressing SlGRAS4 (SlGRAS4-OE) in tomato enhanced tolerance of this stress. Under stress condition SlGRAS4-OE plants accumulated much less ROS than wild-type and SlGRAS4-RNAi plants. Numerous dehydration induced ROS-scavenging genes were upregulated in SlGRAS4-OE plants after drought stress, implying that SlGRAS4 confers drought tolerance by modulating ROS homeostasis. On the other hand, there are several abscisic acid (ABA)-responsive elements in SlGRAS4 promoter, the relative expression of ABA signaling genes including SlPYLs, SlPP2Cs and SlSnRK2s were verified in WT and transgenic plants both under normal and drought stress, the changed drought sensitivity of transgenic plants was mainly caused by SlSnRK2s, the positive regulators of ABA signaling. Our results suggested that SlGRAS4 directly binds to and activates SlSnRK2.4 promoter, belongs to subclass III SnRK2s, which play crucial role in ABA signaling. Protein studies revealed that SlSnRK2.4 interacts with SlAREB1 and SlAREB2, the major downstream transcription factors of ABA-dependent signaling pathway. SlGRAS4 therefore confers drought tolerance may be through SnRK2-AREB pathway.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
