Characterization of the novel mitochondrial genome segregation factor TAP110 in Trypanosoma brucei .

Szczegóły
Abstrakt

Tytuł:: Characterization of the novel mitochondrial genome segregation factor TAP110 in Trypanosoma brucei .
Autorzy:: Amodeo S; Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.
Kalichava A; Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.
Fradera-Sola A; Institute of Molecular Biology, 55128 Mainz, Germany.
Bertiaux-Lequoy E; Department of Cell Biology, University of Geneva, Sciences III, 1211 Geneva, Switzerland.
Guichard P; Department of Cell Biology, University of Geneva, Sciences III, 1211 Geneva, Switzerland.
Butter F; Institute of Molecular Biology, 55128 Mainz, Germany.
Ochsenreiter T; Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland .
Źródło:: Journal of cell science [J Cell Sci] 2021 Mar 08; Vol. 134 (5). Date of Electronic Publication: 2021 Mar 08.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Cambridge : Company of Biologists
Original Publication: London.
MeSH Terms:: Genome, Mitochondrial*/genetics
Trypanosoma brucei brucei*/genetics
DNA, Kinetoplast/genetics ; Mitochondria ; Protozoan Proteins/genetics
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Contributed Indexing:: Keywords: Kinetoplast DNA; Mitochondrial genome segregation machinery; TAC; Tripartite attachment complex; Trypanosoma brucei; U-ExM; Ultrastructure expansion microscopy; kDNA
Substance Nomenclature:: 0 (DNA, Kinetoplast)
0 (Protozoan Proteins)
Entry Date(s):: Date Created: 20210216 Date Completed: 20210712 Latest Revision: 20210712
Update Code:: 20240104
PubMed Central ID:: PMC7970207
DOI:: 10.1242/jcs.254300
PMID:: 33589495
: Czasopismo naukowe

Proper mitochondrial genome inheritance is important for eukaryotic cell survival. Trypanosoma brucei , a protozoan parasite, contains a singular mitochondrial genome, the kinetoplast (k)DNA. The kDNA is anchored to the basal body via the tripartite attachment complex (TAC) to ensure proper segregation. Several components of the TAC have been described; however, the connection of the TAC to the kDNA remains elusive. Here, we characterize the TAC-associated protein TAP110. We find that both depletion and overexpression of TAP110 leads to a delay in the separation of the replicated kDNA networks. Proteome analysis after TAP110 overexpression identified several kDNA-associated proteins that changed in abundance, including a TEX-like protein that dually localizes to the nucleus and the kDNA, potentially linking replication and segregation in the two compartments. The assembly of TAP110 into the TAC region seems to require the TAC but not the kDNA itself; however, once TAP110 has been assembled, it also interacts with the kDNA. Finally, we use ultrastructure expansion microscopy in trypanosomes for the first time, and reveal the precise position of TAP110 between TAC102 and the kDNA, showcasing the potential of this approach.This article has an associated First Person interview with the first author of the paper.
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2021. Published by The Company of Biologists Ltd.)

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