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Tytuł:
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Evidence of thermophilic waste decomposition at a landfill exhibiting elevated temperature regions.
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Autorzy:
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De la Cruz FB; Department of Civil, Construction, and Environmental Engineering, Campus Box 7908, North Carolina State University, Raleigh, NC 27695-7908, United States. Electronic address: .
Cheng Q; Department of Civil, Construction, and Environmental Engineering, Campus Box 7908, North Carolina State University, Raleigh, NC 27695-7908, United States.
Call DF; Department of Civil, Construction, and Environmental Engineering, Campus Box 7908, North Carolina State University, Raleigh, NC 27695-7908, United States.
Barlaz MA; Department of Civil, Construction, and Environmental Engineering, Campus Box 7908, North Carolina State University, Raleigh, NC 27695-7908, United States.
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Źródło:
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Waste management (New York, N.Y.) [Waste Manag] 2021 Apr 01; Vol. 124, pp. 26-35. Date of Electronic Publication: 2021 Feb 14.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: New York : Pergamon Press, c1989-
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MeSH Terms:
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Euryarchaeota*/genetics
Waste Disposal Facilities*
Methane ; RNA, Ribosomal, 16S/genetics ; Temperature
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Contributed Indexing:
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Keywords: Elevated temperature; Landfill; Methanogenic activity; Microbial community
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Substance Nomenclature:
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0 (RNA, Ribosomal, 16S)
OP0UW79H66 (Methane)
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Entry Date(s):
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Date Created: 20210217 Date Completed: 20210316 Latest Revision: 20210316
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Update Code:
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20240104
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DOI:
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10.1016/j.wasman.2021.01.014
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PMID:
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33596536
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There have been several reports of landfills exhibiting temperatures as high as 80 to 100 °C. This observation has motivated researchers to understand the causes of the elevated temperatures and to develop predictive models of landfill temperature. The objective of this research was to characterize the methanogenic activity of microbial communities that were derived from landfill samples excavated from a section of a landfill exhibiting gas well temperatures above 55 °C. Specific objectives were to: (1) determine the upper temperature limit for methane production; (2) evaluate the kinetics of methane generation when landfill-derived microcosms are incubated above and below their excavation temperature and derive a temperature inhibition function; and (3) evaluate microbial community shifts in response to temperature perturbations. Landfill microcosms were derived from 57 excavated landfill samples and incubated within ±2.5 °C of their excavation temperature between 42.5 °C and 87.5 °C. Results showed an optimum temperature for methane generation of ~57 °C and a 95% reduction in methane yield at ~72 °C. When select cultures were perturbed between 5 °C below and 15 °C above their in-situ temperature, both the rate and maximum methane production decreased as incubation temperature increased. Microbial community characterization using 16S rRNA amplicon sequencing suggests that thermophilic methanogenic activity can be attributed to methanogens of the genus Methanothermobacter. This study demonstrated that from a microbiological standpoint, landfills may maintain active methanogenic processes while experiencing temperatures in the thermophilic regime (<72 °C).
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
