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Tytuł:
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Antiviral activity and mechanism of ESC-1GN from skin secretion of Hylarana guentheri against influenza A virus.
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Autorzy:
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Yang J; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Zhang B; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Huang Y; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Liu T; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Zeng B; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Chai J; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Wu J; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
Xu X; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Shatai Nan Road, No. 1023-1063, Guangzhou, Guangdong 510515, China.
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Źródło:
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Journal of biochemistry [J Biochem] 2021 Sep 07; Vol. 169 (6), pp. 757-765.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: : Abingdon, UK : Oxford University Press
Original Publication: Tokyo : Japanese Biochemical Society
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MeSH Terms:
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Virus Internalization*
Amphibian Proteins/*chemistry
Antiviral Agents/*pharmacology
Influenza A virus/*drug effects
Influenza, Human/*drug therapy
Peptide Fragments/*pharmacology
Skin/*metabolism
A549 Cells ; Animals ; Dogs ; Humans ; Influenza, Human/virology ; Madin Darby Canine Kidney Cells ; Ranidae ; Virus Replication
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Grant Information:
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31772476 National Natural Science Foundation of China; 2019A1515011550 Natural Science Foundation of Guangdong Province
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Contributed Indexing:
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Keywords: ESC-1GN; amphibian; antimicrobial peptide; haemagglutinin; influenza A virus
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Substance Nomenclature:
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0 (Amphibian Proteins)
0 (Antiviral Agents)
0 (Peptide Fragments)
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Entry Date(s):
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Date Created: 20210224 Date Completed: 20210928 Latest Revision: 20210928
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Update Code:
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20240105
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DOI:
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10.1093/jb/mvab019
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PMID:
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33624755
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Development of new and effective antiinfluenza drugs is critical for prophylaxis and treatment of influenza A virus (IAV) infection. A wide range of amphibian skin secretions have been identified to show antiviral activity. Our previously reported ESC-1GN, a peptide from the skin secretion of Hylarana guentheri, displayed good antimicrobial and antiinflammatory effects. Here, we found that ESC-1GN possessed significant antiviral effects against IAVs. Moreover, ESC-1GN could inhibit the entry of divergent H5N1 and H1N1 virus strains with the IC50 values from 1.29 to 4.59 μM. Mechanism studies demonstrated that ESC-1GN disrupted membrane fusion activity of IAVs by interaction with HA2 subunit. The results of site-directed mutant assay and molecular docking revealed that E105, N50 and the residues around them on HA2 subunit could form hydrogen bonds with amino acid on ESC-1GN, which were critical for ESC-1GN binding to HA2 and inhibiting the entry of IAVs. Altogether, these not only suggest that ESC-1GN maybe represent a new type of excellent template designing drugs against IAVs, but also it may shed light on the immune mechanism and survival strategy of H.guentheri against viral pathogens.
(© The Author(s) 2021. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.)
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