Enriched Au nanoclusters with mesoporous silica nanoparticles for improved fluorescence/computed tomography dual-modal imaging.

Szczegóły
Abstrakt

Tytuł:: Enriched Au nanoclusters with mesoporous silica nanoparticles for improved fluorescence/computed tomography dual-modal imaging.
Autorzy:: Yuan Y; Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
Zhou R; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Li T; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Qu S; Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
Bai H; Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
Liang J; Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
Cai X; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Guo B; Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
Źródło:: Cell proliferation [Cell Prolif] 2021 Apr; Vol. 54 (4), pp. e13008. Date of Electronic Publication: 2021 Feb 25.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: [Oxford, England] : Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications, 1991-
MeSH Terms:: Microscopy, Confocal*
X-Ray Microtomography*
Biocompatible Materials/*chemistry
Gold/*chemistry
Nanoparticles/*chemistry
Silicon Dioxide/*chemistry
Animals ; Biocompatible Materials/pharmacology ; Cattle ; Cell Line ; Cell Survival/drug effects ; Humans ; Mice ; Nanoparticles/toxicity ; Porosity ; Serum Albumin, Bovine/chemistry
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Grant Information:: 2018YFC1002205 The National Key Research and Development Program of China; 2018M633359 Postdoctoral Science Foundation of China; 2020JDRC0056 Sichuan Science and Technology Program
Contributed Indexing:: Keywords: Au nanoclusters; computed tomography; dual-modal imaging; fluorescence; mesoporous silica nanoparticles
Substance Nomenclature:: 0 (Biocompatible Materials)
27432CM55Q (Serum Albumin, Bovine)
7440-57-5 (Gold)
7631-86-9 (Silicon Dioxide)
Entry Date(s):: Date Created: 20210226 Date Completed: 20210413 Latest Revision: 20210413
Update Code:: 20240105
PubMed Central ID:: PMC8016642
DOI:: 10.1111/cpr.13008
PMID:: 33634540
: Czasopismo naukowe

Pełny tekst

Objectives: Au nanoclusters (AuNCs) have been used widely in fluorescence bio-imaging because of their good fluorescence, small particle size and non-cytotoxicity. AuNCs are also efficient in computed tomography (CT) imaging. Hence, a dual-modal imaging probe can be constructed without any complicated modification processes by exploiting the excellent performance of AuNCs. In the present study, AuNCs were enriched with mesoporous silica nanoparticles (MSNs) to obtain enhanced fluorescence/CT dual-modal imaging, which was capable of acquiring more imaging information for diseases compared with single-mode imaging.
Materials and Methods: Biocompatible bovine serum albumin (BSA)-capped AuNCs were prepared and loaded into amine-functionalized MSNs to form MSN@AuNCs. BSA-AuNCs, MSNs, and MSN@AuNCs were characterized by ultraviolet-visible (UV-vis) spectra, transmission electron microscopy (TEM), fluorescence spectra, and zeta potential. CT imaging was recorded using micro-CT scanning. Fluorescence imaging was measured using confocal laser scanning microscopy and flow cytometry.
Results: The prepared AuNCs and MSNs possessed good properties as previously reported. The fluorescence intensity and CT value of the AuNCs were enhanced after being enriched with MSNs. The nanoparticles were both non-cytotoxic. Confocal laser scanning microscopy and flow cytometry indicated that MSN@AuNCs in CAL-27 cells showed improved fluorescence imaging compared with simple AuNCs at the same concentration.
Conclusions: The results revealed that the strategy of enriching AuNCs with MSNs can obtain highly sensitive fluorescence/CT dual-modal imaging, which indicated the potential of this nanoparticle in the diagnosis and treatment of disease.
(© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.)

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