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Tytuł pozycji:

[Curcumin mediates IL-6/STAT3 signaling pathway to repair intestinal mucosal injury induced by 5-FU chemotherapy for colon cancer].

Tytuł:
[Curcumin mediates IL-6/STAT3 signaling pathway to repair intestinal mucosal injury induced by 5-FU chemotherapy for colon cancer].
Autorzy:
Xu L; Cancer Hospital of University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Hangzhou 310022, China the Second Clinical Medical College of Zhejiang Chinese Medical University Hangzhou 310053, China.
Wang X; Sir Run Run Shaw Hospital Affiliated to Medical College of Zhejiang University Hangzhou 310020, China.
Wang XY; the Second Clinical Medical College of Zhejiang Chinese Medical University Hangzhou 310053, China.
Yao QH; Cancer Hospital of University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Hangzhou 310022, China.
Chen YB; Cancer Hospital of University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Hangzhou 310022, China.
Źródło:
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2021 Feb; Vol. 46 (3), pp. 670-677.
Typ publikacji:
Journal Article
Język:
Chinese
Imprint Name(s):
Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo
Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989-
MeSH Terms:
Colonic Neoplasms*/drug therapy
Curcumin*
Animals ; Fluorouracil/toxicity ; Interleukin-6/genetics ; Intestinal Mucosa/metabolism ; Rats ; Rats, Sprague-Dawley ; STAT3 Transcription Factor/genetics ; STAT3 Transcription Factor/metabolism ; Signal Transduction
Contributed Indexing:
Keywords: 5-fluorouracil(5-FU); IL-6/STAT3 signaling pathway; colon cancer; curcumin; intestinal mucosal injury
Substance Nomenclature:
0 (Interleukin-6)
0 (STAT3 Transcription Factor)
IT942ZTH98 (Curcumin)
U3P01618RT (Fluorouracil)
Entry Date(s):
Date Created: 20210301 Date Completed: 20210302 Latest Revision: 20210302
Update Code:
20240105
DOI:
10.19540/j.cnki.cjcmm.20201106.401
PMID:
33645034
Czasopismo naukowe
This study aims to investigate the potential mechanism of curcumin in mediating interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3) signaling pathway to repair intestinal mucosal injury induced by 5-fluorouracil(5-FU) chemotherapy for colon cancer. SD rats were intraperitoneally injected with 60 mg·kg~(-1)·d~(-1) 5-FU for 4 days to establish a model of intestinal mucosal injury. Then the rats were randomly divided into model group(equal volume of normal saline), curcumin low, medium and high dose groups(50, 100, 200 mg·kg~(-1)), and normal SD rats were used as control group(equal volume of normal saline). Each group received gavage administration for 4 consecutive days, and the changes of body weight and feces were recorded every day. After administration, blood was collected from the heart, and jejunum tissues were collected. The levels of serum interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) were detected by ELISA, and at the same time, the concentration of Evans blue(EB) in jejunum was measured. Hematoxylin-eosin(HE) staining was used to observe the pathological state of jejunum, and the length of jejunum villi and the depth of crypt were measured. The positive expression levels of claudin, occludin and ZO-1 were detected by immunohistochemistry. Western blot was used to detect the protein expression of IL-6, p-STAT3, E-cadherin, vimentin and N-cadherin in jejunum tissues. The results showed that, curcumin significantly increased body weight and fecal weight(P<0.05 or P<0.01), decreased fecal score, EB concentration, IL-1β and TNF-α levels(P<0.05 or P<0.01) in rats. In addition, curcumin maintained the integrity of mucosal surface and villi structure of jejunum to a large extent, and reduced pathological changes in a dose-dependent manner. Meanwhile, curcumin could increase the positive expression of occludin, claudin and ZO-1(P<0.05 or P<0.01), repair intestinal barrier function, downregulate the protein expression of IL-6, p-STAT3, vimentin and N-cadherin in jejunum tissues(P<0.05 or P<0.01), and upregulate the protein expression of E-cadherin(P<0.05). Therefore, curcumin could repair the intestinal mucosal injury induced by 5-FU chemotherapy for colon cancer, and the mechanism may be related to the inhibition of IL-6/STAT3 signal and the inhibition of epithelial-mesenchymal transition(EMT) process.

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