Shotgun Proteomics of Isolated Urinary Extracellular Vesicles for Investigating Respiratory Impedance in Healthy Preschoolers.

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Abstrakt

Tytuł:: Shotgun Proteomics of Isolated Urinary Extracellular Vesicles for Investigating Respiratory Impedance in Healthy Preschoolers.
Autorzy:: Ferrante G; Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, Piazza delle Cliniche, 2, 90127 Palermo, Italy.
Rossi R; National Research Council of Italy, Proteomics and Metabolomics Unit, Institute for Biomedical Technologies, ITB-CNR, Via Fratelli Cervi, 93, Segrate, 20090 Milan, Italy.
Cilluffo G; National Research Council of Italy, Institute for Biomedical Research and Innovation (IRIB), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Di Silvestre D; National Research Council of Italy, Proteomics and Metabolomics Unit, Institute for Biomedical Technologies, ITB-CNR, Via Fratelli Cervi, 93, Segrate, 20090 Milan, Italy.
Brambilla A; Stem Cell Laboratory, Department of Pathophysiology and Transplantation, University of Milan, Unit of Neurology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, 20122 Milan, Italy.
De Palma A; National Research Council of Italy, Proteomics and Metabolomics Unit, Institute for Biomedical Technologies, ITB-CNR, Via Fratelli Cervi, 93, Segrate, 20090 Milan, Italy.
Villa C; Stem Cell Laboratory, Department of Pathophysiology and Transplantation, University of Milan, Unit of Neurology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, 20122 Milan, Italy.
Malizia V; National Research Council of Italy, Institute for Biomedical Research and Innovation (IRIB), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Gagliardo R; National Research Council of Italy, Institute for Biomedical Research and Innovation (IRIB), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Torrente Y; Stem Cell Laboratory, Department of Pathophysiology and Transplantation, University of Milan, Unit of Neurology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, 20122 Milan, Italy.
Corsello G; Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, Piazza delle Cliniche, 2, 90127 Palermo, Italy.
Viegi G; National Research Council of Italy, Institute for Biomedical Research and Innovation (IRIB), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Mauri P; National Research Council of Italy, Proteomics and Metabolomics Unit, Institute for Biomedical Technologies, ITB-CNR, Via Fratelli Cervi, 93, Segrate, 20090 Milan, Italy.; Clinical Proteomics Laboratory c/o ITB-CNR, CNR.Biomics Infrastructure, Elixir, Via Fratelli Cervi, 93, Segrate, 20090 Milan, Italy.
La Grutta S; National Research Council of Italy, Institute for Biomedical Research and Innovation (IRIB), Via Ugo La Malfa 153, 90146 Palermo, Italy.
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2021 Feb 26; Vol. 26 (5). Date of Electronic Publication: 2021 Feb 26.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, c1995-
MeSH Terms:: Proteomics*
Extracellular Vesicles/*genetics
Proteome/*genetics
Urine/*chemistry
Aminopeptidases/urine ; Angiopoietin-Like Protein 2 ; Angiopoietin-like Proteins/urine ; Child, Preschool ; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/urine ; Electric Impedance ; Eye Proteins/urine ; Female ; Humans ; Male ; Nerve Growth Factors/urine ; Proteome/chemistry ; Receptors, Cell Surface/genetics ; Respiratory Function Tests ; Serine Proteases/urine ; Serpins/urine ; Thy-1 Antigens/urine ; Tripeptidyl-Peptidase 1
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Contributed Indexing:: Keywords: extracellular vesicle; forced oscillation technique; preschooler healthy children; proteomics; urine fractionation
Substance Nomenclature:: 0 (ANGPTL2 protein, human)
0 (Angiopoietin-Like Protein 2)
0 (Angiopoietin-like Proteins)
0 (Eye Proteins)
0 (Nerve Growth Factors)
0 (Proteome)
0 (Receptors, Cell Surface)
0 (Serpins)
0 (Thy-1 Antigens)
0 (Tripeptidyl-Peptidase 1)
0 (intrinsic factor-cobalamin receptor)
0 (kallistatin)
0 (pigment epithelium-derived factor)
EC 3.4.- (Serine Proteases)
EC 3.4.11.- (Aminopeptidases)
EC 3.4.14.- (Dipeptidyl-Peptidases and Tripeptidyl-Peptidases)
EC 3.4.14.9 (TPP1 protein, human)
Entry Date(s):: Date Created: 20210303 Date Completed: 20210406 Latest Revision: 20211204
Update Code:: 20240105
PubMed Central ID:: PMC7956503
DOI:: 10.3390/molecules26051258
PMID:: 33652646
: Czasopismo naukowe

Pełny tekst

Urine proteomic applications in children suggested their potential in discriminating between healthy subjects from those with respiratory diseases. The aim of the current study was to combine protein fractionation, by urinary extracellular vesicle isolation, and proteomics analysis in order to establish whether different patterns of respiratory impedance in healthy preschoolers can be characterized from a protein fingerprint. Twenty-one 3-5-yr-old healthy children, representative of 66 recruited subjects, were selected: 12 late preterm (LP) and 9 full-term (T) born. Children underwent measurement of respiratory impedance through Forced Oscillation Technique (FOT) and no significant differences between LP and T were found. Unbiased clustering, based on proteomic signatures, stratified three groups of children (A, B, C) with significantly different patterns of respiratory impedance, which was slightly worse in group A than in groups B and C. Six proteins (Tripeptidyl peptidase I (TPP1), Cubilin (CUBN), SerpinA4, SerpinF1, Thy-1 membrane glycoprotein (THY1) and Angiopoietin-related protein 2 (ANGPTL2)) were identified in order to type the membership of subjects to the three groups. The differential levels of the six proteins in groups A, B and C suggest that proteomic-based profiles of urinary fractionated exosomes could represent a link between respiratory impedance and underlying biological profiles in healthy preschool children.

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