-
Tytuł:
-
Genetic diversity and natural selection on the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum on Bioko Island, Equatorial Guinea and global comparative analysis.
-
Autorzy:
-
Lin LY; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, People's Republic of China.
Huang HY; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China.; Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Liang XY; Department of Medical Laboratory, Huizhou Central Hospital, Huizhou, Guangdong, People's Republic of China.
Xie DD; Department of Medical Laboratory, Foshan Second People's Hospital, Foshan, Guangdong, People's Republic of China.; The Chinese Medical Aid Team To the Republic of Equatorial Guinea, Guangzhou, Guangdong, People's Republic of China.
Chen JT; Department of Medical Laboratory, Huizhou Central Hospital, Huizhou, Guangdong, People's Republic of China.; The Chinese Medical Aid Team To the Republic of Equatorial Guinea, Guangzhou, Guangdong, People's Republic of China.
Wei HG; School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China.
Huang WY; School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China.
Ehapo CS; Department of Medical Laboratory, Malabo Regional Hospital, Malabo, Equatorial Guinea.
Eyi UM; Department of Medical Laboratory, Malabo Regional Hospital, Malabo, Equatorial Guinea.
Li J; Department of Human Parasitology, School of Basic Medical Sciences, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.; Department of Infectious Diseases, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.
Wang JL; School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China.
Zheng YZ; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, People's Republic of China.
Zha GC; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, People's Republic of China.
Wang YL; School of Clinical Medicine, Youjiang Medical University for Nationalities, Baise, China.
Chen WZ; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China.; Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Liu XZ; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China.; Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Mo HT; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China.; Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Chen XY; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China.; Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Lin M; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, People's Republic of China. .; Department of Medical Laboratory, Chaozhou People's Hospital Affiliated to Shantou University Medical College, Chaozhou, Guangdong, People's Republic of China. .; Shantou University Medical College, Shantou, Guangdong, People's Republic of China. .
-
Źródło:
-
Malaria journal [Malar J] 2021 Mar 02; Vol. 20 (1), pp. 124. Date of Electronic Publication: 2021 Mar 02.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: London : BioMed Central, [2002-
-
MeSH Terms:
-
Malaria, Falciparum/*parasitology
Plasmodium falciparum/*genetics
Protozoan Proteins/*genetics
Epitopes ; Equatorial Guinea/epidemiology ; Gene Frequency ; Genetic Variation ; Haplotypes ; Humans ; Malaria Vaccines ; Malaria, Falciparum/epidemiology ; Malaria, Falciparum/immunology ; Plasmodium falciparum/immunology ; Polymorphism, Genetic ; Protozoan Proteins/chemistry ; Protozoan Proteins/immunology ; Selection, Genetic
-
References:
-
Mol Biol Evol. 1999 Jan;16(1):37-48. (PMID: 10331250)
Cell. 1997 Aug 8;90(3):511-22. (PMID: 9267031)
PLoS One. 2014 Feb 28;9(2):e90522. (PMID: 24587387)
PLoS One. 2020 Jan 22;15(1):e0216260. (PMID: 31967991)
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W382-8. (PMID: 15980494)
Nat Commun. 2013;4:2836. (PMID: 24284865)
Malar J. 2020 Jul 13;19(1):245. (PMID: 32660484)
Malar J. 2019 Dec 23;18(1):440. (PMID: 31870353)
Nat Commun. 2019 May 27;10(1):2332. (PMID: 31133635)
Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
Sci Rep. 2018 Apr 26;8(1):6622. (PMID: 29700348)
PLoS One. 2016 Dec 15;11(12):e0167951. (PMID: 27978537)
Malar J. 2019 Sep 18;18(1):317. (PMID: 31533747)
Biochem J. 2004 May 1;379(Pt 3):815-22. (PMID: 14741048)
Mol Biol Evol. 2017 Dec 1;34(12):3299-3302. (PMID: 29029172)
Mol Ther. 2017 Feb 1;25(2):547-559. (PMID: 28153101)
EMBO J. 1995 Aug 15;14(16):3883-94. (PMID: 7664729)
Nat Protoc. 2010 Apr;5(4):725-38. (PMID: 20360767)
PLoS One. 2008 Apr 30;3(4):e2027. (PMID: 18446217)
Bioinformatics. 2014 Oct 15;30(20):2981-2. (PMID: 24996895)
EMBO J. 1993 Jul;12(7):2881-9. (PMID: 8392935)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
Bioinformatics. 2011 Aug 1;27(15):2156-8. (PMID: 21653522)
Infect Genet Evol. 2015 Dec;36:552-556. (PMID: 26325683)
Nat Methods. 2010 Apr;7(4):248-9. (PMID: 20354512)
J Mol Evol. 1995 Feb;40(2):190-226. (PMID: 7699723)
Malar J. 2018 Dec 7;17(1):458. (PMID: 30526609)
PLoS Med. 2004 Nov;1(2):e33. (PMID: 15526058)
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Dec;23(6):677-81. (PMID: 22379826)
Parassitologia. 1993 Jul;35 Suppl:69-72. (PMID: 8233617)
Genetics. 1989 Nov;123(3):585-95. (PMID: 2513255)
Infect Genet Evol. 2020 Jun;80:104197. (PMID: 31954917)
-
Grant Information:
-
2019-GDXK-0031 Key scientific research projects of Guangdong Provincial Department of Education; 2019JJD140052 Guangxi Key Research and Development Foundation; AB18221029 Guangxi Key Research and Development Foundation; CZK20200602 Special technology program of Chaozhou for novel coronavirus infection control; CZK20200603 Special technology program of Chaozhou for novel coronavirus infection control; 2020KZDZX1146 Special research project of Guangdong for Novel coronavirus pneumonia epidemic prevention and contro
-
Contributed Indexing:
-
Keywords: Bioko Island; Genetic diversity; Malaria; Natural selection; Plasmodium falciparum thrombospondin-related adhesive protein (PfTRAP); Vaccine candidate
-
Substance Nomenclature:
-
0 (Epitopes)
0 (Malaria Vaccines)
0 (Protozoan Proteins)
120300-02-9 (thrombospondin-related adhesive protein, protozoan)
-
Entry Date(s):
-
Date Created: 20210303 Date Completed: 20210310 Latest Revision: 20210310
-
Update Code:
-
20240105
-
PubMed Central ID:
-
PMC7922716
-
DOI:
-
10.1186/s12936-021-03664-8
-
PMID:
-
33653360
-
Background: Thrombospondin-related adhesive protein (TRAP) is a transmembrane protein that plays a crucial role during the invasion of Plasmodium falciparum into liver cells. As a potential malaria vaccine candidate, the genetic diversity and natural selection of PfTRAP was assessed and the global PfTRAP polymorphism pattern was described.
Methods: 153 blood spot samples from Bioko malaria patients were collected during 2016-2018 and the target TRAP gene was amplified. Together with the sequences from database, nucleotide diversity and natural selection analysis, and the structural prediction were preformed using bioinformatical tools.
Results: A total of 119 Bioko PfTRAP sequences were amplified successfully. On Bioko Island, PfTRAP shows its high degree of genetic diversity and heterogeneity, with π value for 0.01046 and Hd for 0.99. The value of dN-dS (6.2231, p < 0.05) hinted at natural selection of PfTRAP on Bioko Island. Globally, the African PfTRAPs showed more diverse than the Asian ones, and significant genetic differentiation was discovered by the fixation index between African and Asian countries (Fst > 0.15, p < 0.05). 667 Asian isolates clustered in 136 haplotypes and 739 African isolates clustered in 528 haplotypes by network analysis. The mutations I116T, L221I, Y128F, G228V and P299S were predicted as probably damaging by PolyPhen online service, while mutations L49V, R285G, R285S, P299S and K421N would lead to a significant increase of free energy difference (ΔΔG > 1) indicated a destabilization of protein structure.
Conclusions: Evidences in the present investigation supported that PfTRAP gene from Bioko Island and other malaria endemic countries is highly polymorphic (especially at T cell epitopes), which provided the genetic information background for developing an PfTRAP-based universal effective vaccine. Moreover, some mutations have been shown to be detrimental to the protein structure or function and deserve further study and continuous monitoring.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.
