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Tytuł:
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Evaluation of Equine Infectious Anemia Virus by the Indirect Enzyme-linked Immunosorbent Assay EIA-LAB as Screening Tools in Mexico.
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Autorzy:
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Rodríguez Domínguez MC; Viral and Bacterial Vaccines Producer Enterprise, LABIOFAM, Havana, Cuba.
Montes-de-Oca-Jiménez R; Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Toluca, Mexico. Electronic address: .
Vázquez Chagoyan JC; Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Toluca, Mexico.
Pliego AB; Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Toluca, Mexico.
Varela Guerrero JA; Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Toluca, Mexico.
Coroas González LI; Viral and Bacterial Vaccines Producer Enterprise, LABIOFAM, Havana, Cuba.
Bernabé SL; Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Toluca, Mexico; Research and Advanced Studies in Animal Health Center, Animal Health Diagnosis Department, Serology Laboratory, Toluca, Mexico.
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Źródło:
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Journal of equine veterinary science [J Equine Vet Sci] 2021 Mar; Vol. 98, pp. 103372. Date of Electronic Publication: 2021 Jan 06.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: 2003- : New York : Elsevier
Original Publication: [Wildomar, CA : William E. Jones, c1981-
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MeSH Terms:
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Equine Infectious Anemia*/diagnosis
Horse Diseases*
Infectious Anemia Virus, Equine*
Animals ; Antibodies, Viral ; Cuba ; Enzyme-Linked Immunosorbent Assay/veterinary ; Horses ; Mexico ; Reproducibility of Results ; Sensitivity and Specificity
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Contributed Indexing:
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Keywords: AGID; Diagnostic; EIAV; ELISA; Equine
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Substance Nomenclature:
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0 (Antibodies, Viral)
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Entry Date(s):
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Date Created: 20210305 Date Completed: 20210423 Latest Revision: 20210423
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Update Code:
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20240104
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DOI:
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10.1016/j.jevs.2021.103372
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PMID:
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33663722
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Equine infectious anemia is a worldwide distributed disease that affects the Equide family. Commercial effective vaccine is not available, for that reason control of the disease depends on diagnostic tools. To improve the efficiency of the diagnostic program in Cuba, LABIOFAM Group, developed an indirect enzyme-linked immunosorbent assay (ELISA), ELISA kit, to complement the diagnostic system that currently uses the agar gel immunodiffusion (AGID) kit. The ELISA AIE-LAB Kit was evaluated in a Mexican context, compared with the gold standard test Agar gel immunodiffusion, AGID AIE-LABIOFAM, and commercial AGID kit. The analytical sensitivity was determined using serial dilutions twofold of the positive control serum to establish the range of detected antibodies in relation to the cutoff value of the plate (OD 0.300). A precision study was carried out to evaluate repeatability, intermediate precision, and reproducibility by estimating the standard deviation and coefficient of variation. The precision results were satisfactory and the values of the coefficient of variation were considered adequate to guarantee an excellent consistency of the ELISA AIE-LAB. The diagnostic performance of the ELISA AIE-LAB involved the evaluation of specificity, sensitivity, and concordance in comparison with both AGID tests. The diagnostic sensitivity was 100% and the specificity 97.6%, with a very good degree of concordance (Kappa = 0.9). The results suggest that the ELISA AIE-LAB test could be used in Mexico as a diagnostic system for the detection of specific antibodies against the equine infectious anemia virus, as per current international norms.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
