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Tytuł:
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Response surface methodology optimized electrochemical DNA biosensor based on HAPNPTs/PPY/MWCNTs nanocomposite for detecting Mycobacterium tuberculosis.
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Autorzy:
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Rizi KS; Antimicrobial Resistance Research Center, Department of Medical Bacteriology and Virology, Qaem University Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Hatamluyi B; Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
Rezayi M; Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: .
Meshkat Z; Antimicrobial Resistance Research Center, Department of Medical Bacteriology and Virology, Qaem University Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Sankian M; Division of Immunobiochemistry, Immunology Research Centre, Bu- Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Ghazvini K; Antimicrobial Resistance Research Center, Department of Medical Bacteriology and Virology, Qaem University Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Farsiani H; Antimicrobial Resistance Research Center, Department of Medical Bacteriology and Virology, Qaem University Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Aryan E; Antimicrobial Resistance Research Center, Department of Medical Bacteriology and Virology, Qaem University Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: .
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Źródło:
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Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122099. Date of Electronic Publication: 2021 Jan 11.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
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MeSH Terms:
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Biosensing Techniques*
Mycobacterium tuberculosis*/genetics
Nanocomposites*
Nanotubes, Carbon*
DNA/genetics ; Electrochemical Techniques ; Polymers ; Pyrroles
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Contributed Indexing:
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Keywords: Electrochemical DNA biosensor; Hydroxyapatite nanoparticles; Mycobacterium tuberculosis diagnosis; Polypyrrole; Response surface methodology
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Substance Nomenclature:
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0 (Nanotubes, Carbon)
0 (Polymers)
0 (Pyrroles)
9007-49-2 (DNA)
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Entry Date(s):
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Date Created: 20210307 Date Completed: 20210514 Latest Revision: 20210514
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Update Code:
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20240104
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DOI:
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10.1016/j.talanta.2021.122099
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PMID:
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33676656
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An important issue in the prognosis of tuberculosis (TB) is a short period between correct diagnosis and start the suitable antibiotic therapy. So, a rapid and valid method for detection of Mycobacterium tuberculosis (M. tb) complex is considered as a necessity. Herein, a rapid, low-cost, and PCR-free DNA biosensor was developed based on multi-walled carbon nanotubes (MWCNTs), polypyrrole (PPy), and hydroxyapatite nanoparticles (HAPNPs) for highly sensitive and specific recognition of M.tb. The biosensor consisted of M.tb ssDNA probe covalently attached to the HANPs/PPy/MWCNTs/GCE surface that hybridized to a complementary target sequence to form a duplex DNA. The M.tb target recognition was based on the oxidation signal of the electroactive Methylene Blue (MB) on the surface of the modified GCE using differential pulse voltammetry (DPV) method. It is worth to mention that for the first time Plackett-Burman (PB) screening design and response surface method (RSM) based on central composite design (CCD) was applied as a powerful and an efficient approach to find optimal conditions for maximum M.tb biosensor performance leading to simplicity and rapidity of operation. The proposed DNA biosensor exhibits a wide detection range from 0.25 to 200.0 nM with a low detection limit of 0.141 nM. The performance of designed biosensor for clinical diagnosis and practical applications was revealed through hybridization between DNA probe-modified GCE and extracted DNA from sputum clinical samples.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
