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Tytuł pozycji:

Fluorescence on-off-on with small and charge-tunable nanoparticles enables highly sensitive intracellular microRNA imaging in living cells.

Tytuł :
Fluorescence on-off-on with small and charge-tunable nanoparticles enables highly sensitive intracellular microRNA imaging in living cells.
Autorzy :
Li C; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China; Qiannan Medical College for Nationalities, Duyun, 558000, China.
Zhang Y; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Chen J; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Liu L; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Yang H; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Liu SY; School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.
Xu Y; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Dai Z; School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China. Electronic address: .
Zou X; School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: .
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Źródło :
Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122114. Date of Electronic Publication: 2021 Jan 15.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
MicroRNAs*/genetics
Nanoparticles*
Nanostructures*
Humans ; Limit of Detection ; Spectrometry, Fluorescence
Contributed Indexing :
Keywords: Cell imaging; Charge-tunable; Fluorescent sensor; MicroRNA detection; Silicon nanoparticles
Substance Nomenclature :
0 (MicroRNAs)
Entry Date(s) :
Date Created: 20210307 Date Completed: 20210514 Latest Revision: 20210514
Update Code :
20210515
DOI :
10.1016/j.talanta.2021.122114
PMID :
33676670
Czasopismo naukowe
Nanomaterial-based on-off-on fluorescence sensing strategies are significant particularly in intracellular nucleic acids imaging assay. There still remains challenge to rationally balance fluorescence quenching efficiency and recovery dynamics. We assume that the performance of on-off-on fluorescence sensing strategy can be fundamentally improved on small zero-dimensional (0D) nanomaterial with precisely modulated surface charge. For a proof-of-concept demonstration, silicon nanoparticle (SiNP) with ~4 nm was synthesized and used as the quencher model, of which the surface charge density was modulated by modification of triphenylphosphonium (TPP). The influence of particle size, surface charge and charge density of the nanomaterials on sensing performance was systematically investigated. The strategy showed a low limit of detection (LOD) as 26 pM for target model miR-494, which is one of the lowest in nanomaterial-based on-off-on sensing platforms. And the LOD is even comparable to amplification-based methods in a greatly shortened assay time (2.5 h). The miR-494 expresses in cancerous and normal living cells of human cervical carcinoma (HeLa), human lung carcinoma (A549), human breast cancer (MCF-7), and normal human mammary epithelial (MCF-10A) cells were imaged and localized with significantly improved sensitivity and specificity. These excellent performances insure it a promising candidate as convenient and non-enzymatic sensing platform for miRNA-associated disease detection and early diagnosis.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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