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Title of the item:

Magnetic metal oxide affinity chromatography-based molecularly imprinted approach for effective separation of serous and urinary phosphoprotein biomarker.

Title :
Magnetic metal oxide affinity chromatography-based molecularly imprinted approach for effective separation of serous and urinary phosphoprotein biomarker.
Authors :
Fang X; Department of Gastroenterology and Hepatology, Zhongshan Hospital, And Department of Chemistry, Fudan University, Shanghai, 200032, China.
Wang Z; Department of Gastroenterology and Hepatology, Zhongshan Hospital, And Department of Chemistry, Fudan University, Shanghai, 200032, China.
Sun N; Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. Electronic address: .
Deng C; Department of Gastroenterology and Hepatology, Zhongshan Hospital, And Department of Chemistry, Fudan University, Shanghai, 200032, China. Electronic address: .
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Source :
Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122143. Date of Electronic Publication: 2021 Jan 26.
Publication Type :
Journal Article
Language :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
Molecular Imprinting*
Oxides*
Phosphoproteins/*urine
Biomarkers/urine ; Chromatography, Affinity ; Humans ; Magnetic Phenomena
Contributed Indexing :
Keywords: Body fluid; Molecular imprinting technology; Phosphoprotein biomarker
Substance Nomenclature :
0 (Biomarkers)
0 (Oxides)
0 (Phosphoproteins)
Entry Date(s) :
Date Created: 20210307 Date Completed: 20210514 Latest Revision: 20210621
Update Code :
20210628
DOI :
10.1016/j.talanta.2021.122143
PMID :
33676694
Academic Journal
Many phosphoprotein biomarkers have been proved to exist in body fluids such as serum and urine, however, there is absence of rapid and efficient separation and identification method. In this study, we proposed to combine metal oxide affinity chromatography (MOAC), molecular imprinting technology (MIT) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to establish an effective approach to solve this problem. To verify the feasibility of this approach, we selected a typical phosphoprotein lysozyme (Lys) as template and magnetic TiO 2 as substrate to prepare the molecularly imprinted nanoparticles (denoted as Fe 3 O 4 @TiO 2 @Lys MIPs). A point worth noting is that polydopamine (PDA) as polymer layer made Fe 3 O 4 @TiO 2 @Lys MIPs more hydrophilic and biocompatible. Thanks to the recognition sites of phosphate and the template-shaped cavities, Fe 3 O 4 @TiO 2 @Lys MIPs showed great sensitivity (0.01 ng*μL -1 ) and selectivity (Lysozyme: BSA: β-casein = 1:100:100, mass ratio) in standard phosphoprotein solution. At the end, the Fe 3 O 4 @TiO 2 @Lys MIPs showed great separation ability to lysozyme phosphoprotein in both human serum and urine samples. Therefore, the MOAC-based molecularly imprinted approach is worthy to be expected in effective separation of phosphoprotein biomarker in complex body fluid, which will be a promising one in future.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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