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Tytuł pozycji:

Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing.

Tytuł :
Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing.
Autorzy :
Iwe IA; Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Li W; Biomaterials Research Center, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, 510515, China.
Li Z; Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. Electronic address: .
Huang J; Biomaterials Research Center, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, 510515, China. Electronic address: .
Pokaż więcej
Źródło :
Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122146. Date of Electronic Publication: 2021 Feb 05.
Typ publikacji :
Journal Article; Review
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
Biosensing Techniques*
G-Quadruplexes*
Nucleic Acids*
DNA/genetics ; DNA Probes ; Nucleic Acid Amplification Techniques
Contributed Indexing :
Keywords: Catalytic hairpin assembly; G-quadruplex; Hybridization chain reaction; Isothermal amplification molecular reaction; Nanomaterials; Nuclease
Substance Nomenclature :
0 (DNA Probes)
0 (Nucleic Acids)
9007-49-2 (DNA)
Entry Date(s) :
Date Created: 20210307 Date Completed: 20210514 Latest Revision: 20210514
Update Code :
20210515
DOI :
10.1016/j.talanta.2021.122146
PMID :
33676697
Czasopismo naukowe
Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate nucleic acid targets or enhance the detection signals. Polymerase chain reaction (PCR) is one of the most popular and powerful techniques for nucleic acid analysis. But the requirement of costly devices for precise thermo-cycling procedures in PCR has severely hampered the wide applications of PCR. Fortunately, isothermal molecular reactions have emerged as promising alternatives. The past decade has witnessed significant progress in the research of isothermal molecular reactions utilizing hairpin DNA probes (HDPs). Based on the nucleic acid strand interaction mechanisms, the hairpin DNA-mediated isothermal amplification (HDMIA) techniques can be mainly divided into three categories: strand assembly reactions, strand decomposition reactions, and strand creation reactions. In this review, we introduce the basics of HDMIA methods, including the sensing principles, the basic and advanced designs, and their wide applications, especially those benefiting from the utilization of G-quadruplexes and nanomaterials during the past decade. We also discuss the current challenges encountered, highlight the potential solutions, and point out the possible future directions in this prosperous research area.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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