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Tytuł pozycji:

MnO 2 nanosheet-mediated target-binding-induced FRET strategy for multiplexed microRNAs detection and imaging in living cells.

Tytuł :
MnO 2 nanosheet-mediated target-binding-induced FRET strategy for multiplexed microRNAs detection and imaging in living cells.
Autorzy :
Wang S; School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, Shandong, PR China.
Zhang L; School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, Shandong, PR China.
Kan A; School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, Shandong, PR China.
Xu X; School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, Shandong, PR China.
Zhang N; Department of Oncology, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, PR China. Electronic address: .
Jiang W; School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, Shandong, PR China. Electronic address: .
Pokaż więcej
Źródło :
Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122202. Date of Electronic Publication: 2021 Feb 06.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
Fluorescence Resonance Energy Transfer*
MicroRNAs*/genetics
Cell Line, Tumor ; DNA Probes/genetics ; Humans ; Manganese Compounds ; Oxides
Contributed Indexing :
Keywords: FRET; Multiplexed detection; Simultaneous imaging; microRNA
Substance Nomenclature :
0 (DNA Probes)
0 (Manganese Compounds)
0 (MicroRNAs)
0 (Oxides)
Entry Date(s) :
Date Created: 20210307 Date Completed: 20210514 Latest Revision: 20210621
Update Code :
20210622
DOI :
10.1016/j.talanta.2021.122202
PMID :
33676722
Czasopismo naukowe
In the regulatory network, miRNAs play a regulatory role in a cooperative or antagonistic manner. Simultaneous accurate detection and imaging of multiplexed miRNAs in living cells are of great significance for miRNA-associated biological research and disease diagnosis and treatment. Herein, a MnO 2 nanosheet-mediated target-binding-induced fluorescence resonance energy transfer (FRET) strategy was developed for detection and imaging of multiplexed miRNAs in living cells. Two pairs of DNA probes (P1-AF 488/P1'-Cy3 and P2-AF 488/P2'-AF 594) contained the complementary sequence to target miRNAs (miRNA-373 and miRNA-96) and labelled with different fluorescence dyes were designed. They were adsorbed onto MnO 2 nanosheets by physisorption to form DNA/MnO 2 nanocomposite probes. When the DNA/MnO 2 nanocomposite probes were taken up by cells, the MnO 2 nanosheets were reduced by intracellular glutathione, accompanying the release of DNA probe pairs. Then the DNA probe pairs specifically recognized and combined with miRNA-373 and miRNA-96 to form stable duplexes, respectively, bringing labelled fluorophores into close proximity to occur FRET. Based on this, the simultaneous imaging of miRNA-373 and miRNA-96 in MDA-MB-231 and L02 cells was successfully implemented. The results displayed a higher expression level of target miRNAs in MDA-MB-231 cells compared to L02 cells. The changes in expression levels of miRNA-96 induced by anti-miRNA-96 or mimics in MDA-MB-231 cells could also be monitored. In addition, the ratiometric detections of multiplexed miRNAs were achieved by utilizing the DNA probe pairs. The proposed strategy provides an alternative method for simultaneous accurate detection and imaging of multiplexed miRNAs and has potential application in biomedical applications.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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