-
Tytuł:
-
Advances in sulfur mustard-induced DNA adducts: Characterization and detection.
-
Autorzy:
-
Cheng X; Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, 410073, PR China; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Liu C; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Yang Y; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Liang L; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Chen B; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Yu H; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Xia J; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China.
Liu S; State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, PR China. Electronic address: liu_.
Li Y; Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, 410073, PR China. Electronic address: .
-
Źródło:
-
Toxicology letters [Toxicol Lett] 2021 Jun 15; Vol. 344, pp. 46-57. Date of Electronic Publication: 2021 Mar 08.
-
Typ publikacji:
-
Journal Article; Review
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Amsterdam : Elsevier
Original Publication: Amsterdam, Elsevier/North Holland.
-
MeSH Terms:
-
DNA Adducts*
Chemical Warfare Agents/*toxicity
Mustard Gas/*toxicity
Animals ; Biomarkers/blood ; Humans
-
Contributed Indexing:
-
Keywords: Biomarker; DNA adduct; DNA damage; LC–MS/MS detection; Review; Sample preparation; Sulfur mustard
-
Substance Nomenclature:
-
0 (Biomarkers)
0 (Chemical Warfare Agents)
0 (DNA Adducts)
T8KEC9FH9P (Mustard Gas)
-
Entry Date(s):
-
Date Created: 20210311 Date Completed: 20210421 Latest Revision: 20210421
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.toxlet.2021.03.004
-
PMID:
-
33705862
-
Sulfur mustard (SM) is a blister chemical warfare agent with severe cytotoxicity and genotoxicity. It can extensively alkylate important macromolecules in organisms, such as proteins, DNA, and lipids, and produce a series of metabolites, among which the characteristic ones can be used as biomarkers. The exact toxicological mechanisms of SM remain unclear but mainly involve the DNA lesions induced by alkylation and oxidative stress caused by glutathione depletion. Various methods have been used to analyze DNA damage caused by SM. Among these methods, liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology stands out and makes it possible to observe damage in view of biomarkers induced by SM. Sample preparation is critical for detection by LC-MS/MS and mainly includes DNA isolation, adduct hydrolysis, and adduct purification. Moreover, optimization of chromatographic conditions, selection of MS transitions, and quantitative strategies are also essential. SM-DNA adducts are generally considered to be N 7 -HETEG, O 6 -HETEG, N 7 -BisG, and N 3 -HETEA. This article proposes some other possibilities of SM-DNA adducts for the identification of SM genotoxicity.
Competing Interests: Declaration of Competing Interest We declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
