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Tytuł pozycji:

Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.

Tytuł :
Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.
Autorzy :
Wang L; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
He R; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Lv B; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Yu X; Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei, PR China. Electronic address: .
Liu Y; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Yang J; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Li W; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Wang Y; Medical College of Hubei University of Arts and Sciences, Xiangyang, Hubei, PR China. Electronic address: .
Zhang H; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Yan G; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Mao W; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Liu L; Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei, PR China. Electronic address: .
Wang F; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
Ma L; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, PR China. Electronic address: .
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Źródło :
Talanta [Talanta] 2021 May 15; Vol. 227, pp. 122154. Date of Electronic Publication: 2021 Feb 11.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
Argonaute Proteins/*chemistry
DNA, Viral/*analysis
Ligase Chain Reaction/*methods
Pyrococcus furiosus/*enzymology
RNA, Viral/*analysis
Alphapapillomavirus/chemistry ; Alphapapillomavirus/isolation & purification ; COVID-19/diagnosis ; DNA, Viral/chemistry ; Humans ; Limit of Detection ; Mutation ; Papillomavirus Infections/diagnosis ; RNA, Viral/chemistry ; SARS-CoV-2/chemistry ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/genetics
References :
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Contributed Indexing :
Keywords: HPV; Ligase chain reaction; Nucleic acid detection; Prokaryotic argonaute; SARS-CoV-2
Substance Nomenclature :
0 (Argonaute Proteins)
0 (DNA, Viral)
0 (RNA, Viral)
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
Entry Date(s) :
Date Created: 20210314 Date Completed: 20210323 Latest Revision: 20210323
Update Code :
20210324
PubMed Central ID :
PMC7875706
DOI :
10.1016/j.talanta.2021.122154
PMID :
33714462
Czasopismo naukowe
Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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