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Tytuł pozycji:

Molecular diagnostic of toxigenic Corynebacterium diphtheriae strain by DNA sensor potentially suitable for electrochemical point-of-care diagnostic.

Tytuł :
Molecular diagnostic of toxigenic Corynebacterium diphtheriae strain by DNA sensor potentially suitable for electrochemical point-of-care diagnostic.
Autorzy :
Marchlewicz K; Warsaw University of Technology, Faculty of Chemistry, The Chair of Medical Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland.
Ostrowska I; Warsaw University of Technology, Faculty of Chemistry, The Chair of Medical Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland.
Oszwałdowski S; Faculty of Chemistry, The Chair of Analytical Chemistry, Warsaw University of Technology, 00-664, Warsaw, Poland.
Zasada A; National Institute of Public Health - National Institute of Hygiene, Department of Sera and Vaccines Evaluation, Chocimska 24, 00-791, Warsaw, Poland.
Ziółkowski R; Warsaw University of Technology, Faculty of Chemistry, The Chair of Medical Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland. Electronic address: .
Malinowska E; Warsaw University of Technology, Faculty of Chemistry, The Chair of Medical Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland; CEZAMAT PW, Poleczki 19, 02-822, Warsaw, Poland.
Pokaż więcej
Źródło :
Talanta [Talanta] 2021 May 15; Vol. 227, pp. 122161. Date of Electronic Publication: 2021 Feb 08.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms :
Corynebacterium diphtheriae*/genetics
DNA ; Diphtheria Toxin ; Humans ; Pathology, Molecular ; Point-of-Care Testing
Contributed Indexing :
Keywords: DNA; Diethyldithiocarbamate; Diphtheria diagnostics; Electrochemistry
Substance Nomenclature :
0 (Diphtheria Toxin)
9007-49-2 (DNA)
Entry Date(s) :
Date Created: 20210314 Date Completed: 20210514 Latest Revision: 20210514
Update Code :
20210515
DOI :
10.1016/j.talanta.2021.122161
PMID :
33714465
Czasopismo naukowe
The presented study is focused on the development of electrochemical genosensor for detection of tox gene fragment of toxigenic Corynebacterium diphtheriae strain. Together with our previous studies it fulfils the whole procedure for fast and accurate diagnostic of diphtheria at its early stage of infection with the use of electrochemical methods. The developed DNA sensor potentially can be used in more sophisticated portable device. After the electrochemical stem-loop probe structure optimization the conditions for real asymmetric PCR (aPCR) product detection were selected. As was shown it was crucial to optimize the magnesium and organic solvent concentrations in detection buffer. Under optimal conditions it was possible to selectively detect as low as 20.8 nM of complementary stand in 5 min or 0.5 nM in 30 min with sensitivity of 12.81 and 0.24 1⋅μM -1 respectively. The unspecific biosensor response was elucidated with the use of new electrode blocking agent, diethyldithiocarbamate. Its application in electrochemical genosensors lead to significant higher current values and the biosensor response even in conditions with magnesium ion depletion. The developed biosensor selectivity was examined using samples containing genetic material originated from a number of non-target bacterial species which potentially can be present in the human upper respiratory tract.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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