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Tytuł pozycji:

A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.

Tytuł :
A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.
Autorzy :
Wang H; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Xiao Q; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Wei C; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; College of Life Science, Zhaoqing University, Zhaoqing, 526061, People's Republic of China.
Chen H; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Chen X; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Dai C; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Wen J; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Ma C; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Tu J; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Fu T; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Shen J; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. .
Yi B; National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. .
Pokaż więcej
Źródło :
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2021 May; Vol. 134 (5), pp. 1377-1386. Date of Electronic Publication: 2021 Mar 16.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Berlin, New York, Springer
References :
Arakawa T, Matsunaga M, Matsui K, Itoh K, Kuroda Y, Matsuhira H, Kitazaki K, Kubo T (2020) The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.). BMC Plant Biol 20:503. (PMID: 331436457607634)
Barkan A, Small I (2014) Pentatricopeptide repeat proteins in plants. Annu Rev Plant Biol 65:415.
Barkan A, Rojas M, Fujii S, Yap A, Chong YS, Bond CS, Small I (2012) A combinatorial amino acid code for RNA recognition by pentatricopeptide repeat proteins. Plos Genet 8:e1002910. (PMID: 229160403420917)
Bentolila S, Alfonso AA, Hanson MR (2002) A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants. P Natl Acad Sci USA 99:10887–10892.
Brown GG, Formanova N, Jin H, Wargachuk R, Dendy C, Patil P, Laforest M, Zhang JF, Cheung WY, Landry BS (2003) The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35:262–272. (PMID: 12848830)
Castillo A, Atienza SG, Martin AC (2014) Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome. J Exp Bot 65:6667–6677. (PMID: 252712604246193)
Chen LT, Liu YG (2014) Male sterility and fertility restoration in crops. Annu Rev Plant Biol 65:579–606.
Chen ZW, Zhao N, Li SS, Grover CE, Nie HS, Wendel JF, Hua JP (2017) Plant mitochondrial genome evolution and cytoplasmic male sterility. Crit Rev Plant Sci 36:55–69.
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743. (PMID: 10069079)
Cui X, Wise RP, Schnable PS (1996) The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize. Science 272:1334–1336. (PMID: 8650543)
Dahan J, Mireau H (2013) The Rf and Rf-like PPR in higher plants, a fast-evolving subclass of PPR genes. RNA Biol 10:1469–1476. (PMID: 38584303858430)
Deblock M, Debrouwer D, Tenning P (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol 91:694–701.
Desloire S, Gherbi H, Laloui W, Marhadour S, Clouet V, Cattolico L, Falentin C, Giancola S, Renard M, Budar F, Small I, Caboche M, Delourme R, Bendahmane A (2003) Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family. EMBO Rep 4:588–594. (PMID: 13191981319198)
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349. (PMID: 20309572030957)
Fan Z, Stefansson BR (1986) Influence of temperature on male sterility of two CMS in B. napus. Can J Plant Sci 66:221–227.
Fujii S, Toriyama K (2009) Suppressed expression of RETROGRADE-REGULATED MALE STERILITY restores pollen fertility in cytoplasmic male sterile rice plants. P Natl Acad Sci USA 106:9513–9518.
Fujii S, Bond CS, Small ID (2011) Selection patterns on restorer-like genes reveal a conflict between nuclear and mitochondrial genomes throughout angiosperm evolution. P Natl Acad Sci USA 108:1723–1728.
Fujii S, Kazama T, Ito Y, Kojima S, Toriyama K (2014) A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice. Rice 7:21. (PMID: 262245524884035)
Gillman JD, Bentolila S, Hanson MR (2007) The petunia restorer of fertility protein is part of a large mitochondrial complex that interacts with transcripts of the CMS-associated locus. Plant J 49:217–227. (PMID: 17156410)
Gully BS, Cowieson N, Stanley WA, Shearston K, Small ID, Barkan A, Bond CS (2015) The solution structure of the pentatricopeptide repeat protein PPR10 upon binding atpH RNA. Nucleic Acids Res 43:1918–1926. (PMID: 256096984330388)
He W, Chen C, Adedze YMN, Dong X, Xi K, Sun Y, Dang T, Jin D (2020) Multicentric origin and diversification of atp6-orf79-like structures reveal mitochondrial gene flows in Oryza rufipogon and Oryza sativa. Evol Appl 13:2284–2299. (PMID: 330052247513716)
Heng SP, Shi DY, Hu ZH, Huang T, Li JP, Liu LY, Xia CX, Yuan ZZ, Xu YJ, Fu TD, Wan ZJ (2015) Characterization and classification of one new cytoplasmic male sterility (CMS) line based on morphological, cytological and molecular markers in non-heading Chinese cabbage (Brassica rapa L.). Plant Cell Rep 34:1529–1537. (PMID: 25972263)
Heng SP, Gao J, Wei C, Chen FY, Li XW, Wen J, Yi B, Ma CZ, Tu JX, Fu TD, Shen JX (2018) Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea. J Exp Bot 69:455–466. (PMID: 293010155853284)
Heng SP, Chen FY, Wei C, Li XW, Yi B, Ma CZ, Tu JX, Shen JX, Fu TD, Wen J (2019) Cytological and iTRAQ-based quantitative proteomic analyses of hau CMS in Brassica napus L. J Proteomics 193:230–238. (PMID: 30385414)
Hu J, Wang K, Huang WC, Liu G, Gao Y, Wang JM, Huang Q, Ji YX, Qin XJ, Wan L, Zhu RS, Li SQ, Yang DC, Zhu YG (2012) The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162. Plant Cell 24:109–122. (PMID: 222472523289560)
Huang WC, Hu J, Yu CC, Huang Q, Wan L, Wang LL, Qin XJ, Ji YX, Zhu RS, Li SQ, Zhu YG (2012) Two non-allelic nuclear genes restore fertility in a gametophytic pattern and enhance abiotic stress tolerance in the hybrid rice plant. Theor Appl Genet 124:799–807. (PMID: 22139140)
Huang WC, Yu CC, Hu J, Wang LL, Dan ZW, Zhou W, He CL, Zeng YF, Yao GX, Qi JZ, Zhang ZH, Zhu RS, Chen XF, Zhu YG (2015) Pentatricopeptide-repeat family protein RF6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility. P Natl Acad Sci USA 112:14984–14989.
Huang SB, Van Aken O, Schwarzlander M, Belt K, Millar AH (2016) The roles of mitochondrial reactive oxygen species in cellular signaling and stress response in plants. Plant Physiol 171:1551–1559. (PMID: 270211894936549)
Jaqueth JS, Hou Z, Zheng P, Ren R, Nagel BA, Cutter G, Niu X, Vollbrecht E, Greene TW, Kumpatla SP (2020) Fertility restoration of maize CMS-C altered by a single amino acid substitution within the Rf4 bHLH transcription factor. Plant J 101:101–111. (PMID: 31487408)
Jing B, Heng SP, Tong D, Wan ZJ, Fu TD, Tu JX, Ma CZ, Yi B, Wen J, Shen JX (2012) A male sterility-associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development. J Exp Bot 63:1285–1295. (PMID: 22090439)
Kazama T, Itabashi E, Fujii S, Nakamura T, Toriyama K (2016) Mitochondrial ORF79 levels determine pollen abortion in cytoplasmic male sterile rice. Plant J 85:707–716. (PMID: 26850149)
Kim YJ, Zhang DB (2018) Molecular control of male fertility for crop hybrid breeding. Trends Plant Sci 23:53–65. (PMID: 29126789)
Koizuka N, Imai R, Fujimoto H, Hayakawa T, Kimura Y, Kohno-Murase J, Sakai T, Kawasaki S, Imamura J (2003) Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J 34:407–415. (PMID: 12753581)
Levings CS (1993) Thoughts on cytoplasmic male-sterility in CMS-T maize. Plant Cell 5:1285–1290. (PMID: 12271028160361)
Li Z, Ding B, Zhou X, Wang GL (2017) The rice dynamin-related protein OsDRP1E negatively regulates programmed cell death by controlling the release of cytochrome c from mitochondria. PLoS Pathog 13:e1006157. (PMID: 280812685266325)
Liu Z, Yang ZH, Wang X, Li KD, An H, Liu J, Yang GS, Fu TD, Yi B, Hong DF (2016) A mitochondria-targeted PPR protein restores pol cytoplasmic male sterility by reducing orf224 transcript levels in oilseed rape. Mol Plant 9:1082–1084. (PMID: 27102212)
Liu Z, Dong FM, Wang X, Wang T, Su R, Hong DF, Yang GS (2017) A pentatricopeptide repeat protein restores nap cytoplasmic male sterility in Brassica napus. J Exp Bot 68:4115–4123. (PMID: 289227645853434)
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 method. Methods 25:402–408. (PMID: 11846609)
Luo DP, Xu H, Liu ZL, Guo JX, Li HY, Chen LT, Fang C, Zhang QY, Bai M, Yao N, Wu H, Wu H, Ji CH, Zheng HQ, Chen YL, Ye S, Li XY, Zhao XC, Li RQ, Liu YG (2013) A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet 45:573–577. (PMID: 23502780)
Melonek J, Zhou R, Bayer PE, Edwards D, Stein N, Small I (2019) High intraspecific diversity of Restorer-of-fertility-like genes in barley. Plant J 97:281–295. (PMID: 30276910)
Miranda RG, Rojas M, Montgomery MP, Gribbin KP, Barkan A (2017) RNA-binding specificity landscape of the pentatricopeptide repeat protein PPR10. RNA 23:586–599. (PMID: 281085205340921)
Ning L, Wang H, Li D, Li Y, Chen K, Chao H, Li H, He J, Li M (2020) Genome-wide identification of the restorer-of-fertility-like (RFL) gene family in Brassica napus and expression analysis in Shaan2A cytoplasmic male sterility. BMC Genomics 21:765. (PMID: 331481777641866)
Ogura H (1968) Studies on the new male-sterility in Japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78.
Qin X, Huang Q, Xiao H, Zhang Q, Ni C, Xu Y, Liu G, Yang D, Zhu Y, Hu J (2016) The rice DUF1620-containing and WD40-like repeat protein is required for the assembly of the restoration of fertility complex. New Phytol 210:934–945. (PMID: 26781807)
Rojas M, Yu Q, Williams-Carrier R, Maliga P, Barkan A (2019) Engineered PPR proteins as inducible switches to activate the expression of chloroplast transgenes. Nat Plants 5:505–511. (PMID: 31036912)
Spier A, Stavru F, Cossart P (2019) Interaction between intracellular bacterial pathogens and host cell mitochondria. Microbiol Spectr 7:1–13.
Tang HW, Luo DP, Zhou DG, Zhang QY, Tian DS, Zheng XM, Chen LT, Liu YG (2014) The rice restorer Rf4 for wild-abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts. Mol Plant 7:1497–1500. (PMID: 24728538)
Tang HW, Zheng XM, Li CL, Xie XR, Chen YL, Chen LT, Zhao XC, Zheng HQ, Zhou JJ, Ye S, Guo JX, Liu YG (2017) Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes. Cell Res 27:130–146. (PMID: 27725674)
Touzet P, Meyer EH (2014) Cytoplasmic male sterility and mitochondrial metabolism in plants. Mitochondrion 19:166–171. (PMID: 24769053)
Tuteja R, Saxena RK, Davila J, Shah T, Chen WB, Xiao YL, Fan GY, Saxena KB, Alverson AJ, Spillane C, Town C, Varshney RK (2013) Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome dequencing of four cajanus genotypes. DNA Res 20:485–495. (PMID: 237928903789559)
Wan ZJ, Jing B, Tu JX, Ma CZ, Shen JX, Yi B, Wen J, Huang T, Wang XJ, Fu TD (2008) Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus. Theor Appl Genet 116:355–362. (PMID: 18034224)
Wan ZJ, Shi DY, Zou RC, Huang Y, Bie ZL, Shi Z, Xu YJ, Fu TD (2014) Development and utilization of one new cytoplasmic male sterile line of Chinese leaf mustard (Brassica juncea var. rugosa Bailey). Sci Hortic-Amsterdam 165:211–217.
Wang ZH, Zou YJ, Li XY, Zhang QY, Chen L, Wu H, Su DH, Chen YL, Guo JX, Luo D, Long YM, Zhong Y, Liu YG (2006) Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell 18:676–687. (PMID: 164891231383642)
Wang K, Gao F, Ji YX, Liu Y, Dan ZW, Yang PF, Zhu YG, Li SQ (2013) ORFH79 impairs mitochondrial function via interaction with a subunit of electron transport chain complex III in Honglian cytoplasmic male sterile rice. New Phytol 198:408–418. (PMID: 23437825)
Wei C, Wang HD, Heng SP, Wen J, Yi B, Ma CZ, Tu JX, Shen JX, Fu TD (2019) Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus. Theor Appl Genet 132:2525–2539. (PMID: 31165223)
Wurschum T, Leiser WL, Weissmann S, Maurer HP (2017) Genetic architecture of male fertility restoration of Triticum timopheevii cytoplasm and fine-mapping of the major restorer locus Rf3 on chromosome 1B. Theor Appl Genet 130:1253–1266. (PMID: 28326434)
Xie F, Yuan JL, Li YX, Wang CJ, Tang HY, Xia JH, Yang QY, Wan ZJ (2018) Transcriptome analysis reveals candidate genes associated withleaf etiolation of a cytoplasmic male sterility line in Chinese cabbage (Brassica rapa L. ssp. Pekinensis). Int J Mol Sci 19:922. (PMID: 5979472)
Xu G, Zhong X, Shi Y, Liu Z, Jiang N, Liu J, Ding B, Li Z, Kang H, Ning Y, Liu W, Guo Z, Wang GL, Wang X (2020) A fungal effector targets a heat shock-dynamin protein complex to modulate mitochondrial dynamics and reduce plant immunity. Sci Adv 6:48.
Yamagishi H, Bhat SR (2014) Cytoplasmic male sterility in Brassicaceae crops. Breed Sci 64:38–47. (PMID: 249872894031109)
Yi B, Chen YN, Lei SL, Tu JX, Fu TD (2006) Fine mapping of the recessive genic male-sterile gene (Bnms1) in Brassica napus L. Theor Appl Genet 113:643–650. (PMID: 16804725)
Yin P, Li Q, Yan C, Liu Y, Liu J, Yu F, Wang Z, Long J, He J, Wang HW, Wang J, Zhu JK, Shi Y, Yan N (2013) Structural basis for the modular recognition of single-stranded RNA by PPR proteins. Nature 504:168–171. (PMID: 24162847)
Yoo SD, Cho YH, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2:1565–1572. (PMID: 17585298)
Zhang HG, Che JL, Ge YS, Pei Y, Zhang LJ, Liu QQ, Gu MH, Tang SZ (2017) Ability of Rf5 and Rf6 to restore fertility of Chinsurah Boro II-type cytoplasmic male sterile Oryza sativa (ssp. Japonica) lines. Rice 10:2. (PMID: 281104605253138)
Zhao N, Li Z, Zhang L, Yang X, Mackenzie SA, Hu Z, Zhang M, Yang J (2021) MutS HOMOLOG1 mediates fertility reversion from cytoplasmic male sterile Brassica juncea in response to environment. Plant Cell Environ 44:234–246. (PMID: 32978825)
Grant Information :
31930032 National Natural Science Foundation of China; 31871701 National Natural Science Foundation of China; 2016YFD0100804 National Key Research and Development Program of China
Entry Date(s) :
Date Created: 20210316 Latest Revision: 20210429
Update Code :
20210506
DOI :
10.1007/s00122-021-03777-3
PMID :
33725137
Czasopismo naukowe
Key Message: A mitochondria-localized pentatricopeptide repeat protein was identified by positional cloning and transferred into the hau CMS line, where it successfully restored fertility Cytoplasmic male sterility (CMS) is a maternally inherited trait that can be controlled by restorer-of-fertility (Rf) genes present in the nucleus. The hau CMS was identified as a new form of CMS associated with the mitochondrial transcript orf288; however, a lack of a restorer gene has limited its utilization in Brassica crops. Here, the combination of Brassica 60 K array with bulk segregant analysis and map-based cloning was used to delimit the Rfh locus to an 82.2-kb region on chromosome A09. A candidate gene encoding a mitochondria-localized pentatricopeptide repeat (PPR) protein was identified and transferred into the hau CMS line, where it successfully restored the fertility of the hau CMS plants. Furthermore, the expression analysis showed that Rfh was highly expressed in the flower buds, and the sequence analysis results implied that functional divergence between RFH and rfh could be due to 59 amino acid residue differences in the deduced protein sequences. In addition, a co-separated molecular marker was developed based on the divergent sequences between the dominant and recessive alleles. These results will help enable the heterosis of Brassica crops in the future.

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